Dr. Tushar Kant Beuria

Academics

Degree	University/Institution
Ph.D.	School of Biosciences and Bioengineering, IIT Bombay.
MSc. (Organic Chemistry)	Ravenshaw College, Cuttack

Work Experience

Position	University/Organisation	Period
Scientist-F	Inistitute of Life Sciences, Bhubaneswar	2024 - Present
Scientist-E	Inistitute of Life Sciences, Bhubaneswar	2019 – 2023
Scientist-D	Inistitute of Life Sciences, Bhubaneswar	2015 – 2018
Ramalingaswami Fellow Scientist	Inistitute of Life Sciences, Bhubaneswar	2011-2016
Post-Doctoral Fellow	University of Texas Health Science Center at Houston	2006-2011

Awards & Recognisation

Details
2011: Ramalingaswami fellowship from Department of Biotechnology, Ministry of Science and Technology, Govt. of India 2004 – 2006: Awarded Senior Research Fellowship sponsored by CSIR, India. 2002 – 2004: Awarded Junior Research Fellowship sponsored by CSIR, India.

Research

Details
Laboratory of Bacterial Cell Division and Antimicrobial Resistance Research: 1. Bacterial Cell Division Machinery Understanding the molecular mechanisms of bacterial cell division has been a central focus of my laboratory’s research programme. Bacterial cell division is an essential and highly conserved process orchestrated by the divisome, a supramolecular machine anchored by FtsZ. This prokaryotic tubulin homolog polymerises into a dynamic Z-ring at the midcell. Given that FtsZ and its associated proteins are highly conserved across most bacteria yet absent in humans, the divisome represents a compelling and largely underexploited target for novel antibacterial drug discovery. Over the years, my group has mapped critical protein-protein interaction interfaces within the divisome, characterised the FtsZ interdomain cleft as a druggable vulnerability, defined FtsEX-dependent coordination of septal peptidoglycan synthesis, and identified novel small-molecule binding sites on FtsZ, including unexpected interactions with Doxorubicin (Biochem J, 2015) and aztreonam’s targeting of ZapE (FEBS Lett, 2023). Most recently, we demonstrated that MinDE independently governs bacterial flagellation beyond its canonical division role (J Biol Chem, 2024), expanding our understanding of Min protein biology. Our current focus is on exploiting these mechanistic insights to develop potent, selective inhibitors of divisome assembly as next-generation antibacterial agents. 2. Efflux Pump-Mediated Antimicrobial Resistance & Biofilm Biology Combating multidrug resistance in Staphylococcus aureus is a major research priority for my laboratory, driven by the urgent clinical need for new strategies against this globally significant pathogen. S. aureus employs efflux pumps, molecular machines that actively remove antibiotics before they reach their intracellular targets, and forms robust biofilms, structured, matrix-encased microbial communities that dramatically reduce antibiotic penetration and shield bacteria from host immunity as two of its most formidable resistance strategies. Recognizing that these mechanisms frequently co-exist in clinical isolates, my laboratory investigates both in an integrated drug repurposing framework. Targeting efflux pumps, we have demonstrated that Montelukast, an FDA-approved leukotriene receptor antagonist, suppresses NorB-mediated efflux by facilitating MgrA phosphorylation, restoring antibiotic susceptibility in MDR S. aureus, and validated in a murine infection model (EBioMedicine, 2025). Extending this, we have also shown that the antifungal azoles Sertaconazole and Oxiconazole inhibit both MFS and ABC-type efflux pumps by disrupting the proton motive force, significantly enhancing intracellular antibiotic accumulation and reducing bacterial burden in vivo (Antimicrob Agents Chemother, 2025). Targeting biofilm formation, through screening of the LOPAC library, we have identified 10058-F4 as a potent anti-biofilm compound that achieves >70% inhibition of S. aureus biofilm formation without affecting bacterial viability (Biofilm, 2025). Our current focus is on the molecular and regulatory basis of S. aureus biofilm development, to identify dual-action compounds that simultaneously inhibit efflux and dismantle biofilm architecture, a strategy we believe holds the greatest promise for clinically translatable outcomes against drug-resistant S. aureus infections. 3. Persister Cell Biology Persister cells represent one of the most clinically underappreciated yet consequential drivers of treatment failure in bacterial infections. These phenotypically tolerant, slow-growing subpopulations survive antibiotic challenge without acquiring genetic resistance mutations and serve as the principal reservoir for chronic, recurrent, and relapsing infections, a problem that conventional antibiotics, designed to target actively dividing bacteria, are fundamentally ill-equipped to resolve. My laboratory is deeply interested in the molecular switches that govern persister formation, maintenance, and resuscitation, with a particular focus on toxin-antitoxin (TA) systems as master regulators of this dormant state. We demonstrated that the MazEF TA system interacts mechanistically with rifampicin to selectively inhibit persister survival, revealing a new conceptual framework for antibiotic-TA system crosstalk (BMC Mol Cell Biol, 2020). Building on this foundation, our current focus is to dissect the regulatory architecture of persistence in S. aureus and to develop therapeutic strategies that target the dormant persister reservoir, an approach we believe is essential for achieving durable clinical cure.

Details

Laboratory of Bacterial Cell Division and Antimicrobial Resistance

Research:

1. Bacterial Cell Division Machinery

Understanding the molecular mechanisms of bacterial cell division has been a central focus of my laboratory’s research programme. Bacterial cell division is an essential and highly conserved process orchestrated by the divisome, a supramolecular machine anchored by FtsZ. This prokaryotic tubulin homolog polymerises into a dynamic Z-ring at the midcell. Given that FtsZ and its associated proteins are highly conserved across most bacteria yet absent in humans, the divisome represents a compelling and largely underexploited target for novel antibacterial drug discovery. Over the years, my group has mapped critical protein-protein interaction interfaces within the divisome, characterised the FtsZ interdomain cleft as a druggable vulnerability, defined FtsEX-dependent coordination of septal peptidoglycan synthesis, and identified novel small-molecule binding sites on FtsZ, including unexpected interactions with Doxorubicin (Biochem J, 2015) and aztreonam’s targeting of ZapE (FEBS Lett, 2023). Most recently, we demonstrated that MinDE independently governs bacterial flagellation beyond its canonical division role (J Biol Chem, 2024), expanding our understanding of Min protein biology. Our current focus is on exploiting these mechanistic insights to develop potent, selective inhibitors of divisome assembly as next-generation antibacterial agents.

2. Efflux Pump-Mediated Antimicrobial Resistance & Biofilm Biology

Combating multidrug resistance in Staphylococcus aureus is a major research priority for my laboratory, driven by the urgent clinical need for new strategies against this globally significant pathogen. S. aureus employs efflux pumps, molecular machines that actively remove antibiotics before they reach their intracellular targets, and forms robust biofilms, structured, matrix-encased microbial communities that dramatically reduce antibiotic penetration and shield bacteria from host immunity as two of its most formidable resistance strategies. Recognizing that these mechanisms frequently co-exist in clinical isolates, my laboratory investigates both in an integrated drug repurposing framework. Targeting efflux pumps, we have demonstrated that Montelukast, an FDA-approved leukotriene receptor antagonist, suppresses NorB-mediated efflux by facilitating MgrA phosphorylation, restoring antibiotic susceptibility in MDR S. aureus, and validated in a murine infection model (EBioMedicine, 2025). Extending this, we have also shown that the antifungal azoles Sertaconazole and Oxiconazole inhibit both MFS and ABC-type efflux pumps by disrupting the proton motive force, significantly enhancing intracellular antibiotic accumulation and reducing bacterial burden in vivo (Antimicrob Agents Chemother, 2025). Targeting biofilm formation, through screening of the LOPAC library, we have identified 10058-F4 as a potent anti-biofilm compound that achieves >70% inhibition of S. aureus biofilm formation without affecting bacterial viability (Biofilm, 2025). Our current focus is on the molecular and regulatory basis of S. aureus biofilm development, to identify dual-action compounds that simultaneously inhibit efflux and dismantle biofilm architecture, a strategy we believe holds the greatest promise for clinically translatable outcomes against drug-resistant S. aureus infections.

3. Persister Cell Biology

Persister cells represent one of the most clinically underappreciated yet consequential drivers of treatment failure in bacterial infections. These phenotypically tolerant, slow-growing subpopulations survive antibiotic challenge without acquiring genetic resistance mutations and serve as the principal reservoir for chronic, recurrent, and relapsing infections, a problem that conventional antibiotics, designed to target actively dividing bacteria, are fundamentally ill-equipped to resolve. My laboratory is deeply interested in the molecular switches that govern persister formation, maintenance, and resuscitation, with a particular focus on toxin-antitoxin (TA) systems as master regulators of this dormant state. We demonstrated that the MazEF TA system interacts mechanistically with rifampicin to selectively inhibit persister survival, revealing a new conceptual framework for antibiotic-TA system crosstalk (BMC Mol Cell Biol, 2020). Building on this foundation, our current focus is to dissect the regulatory architecture of persistence in S. aureus and to develop therapeutic strategies that target the dormant persister reservoir, an approach we believe is essential for achieving durable clinical cure.

Publications

Details
2026 Efflux-Mediated Resistance in Staphylococcus aureus: Challenges and Opportunities for Therapeutic Intervention Ojha S, Beuria TK, *Indian Journal of Microbiology*, 1–30 (2026), DOI: 10.1007/s12088-026-01545-9 2025 Efflux pump modulation by Montelukast and its roles in restoring antibiotic susceptibility in multidrug-resistant Staphylococcus aureus Ojha S, Sinsinwar S, Chatterjee P, Biswal S, Pradhan P, Beuria TK, *EBioMedicine*, 114, 105658 (2025) PubMed: 40157128 10058-F4 Mediated inhibition of the biofilm formation in multidrug-resistant Staphylococcus aureus Dodia H, Ojha S, Chatterjee P, Beuria TK, *Biofilm*, 8, 100307 (2025), DOI: 10.1016/j.bioflm.2025.100307 Inhibition of efflux pumps by FDA-approved drugs oxiconazole and sertaconazole restores antibiotic susceptibility in multidrug-resistant S. aureus Ojha S, Chatterjee P, Beuria TK, *Antimicrobial Agents and Chemotherapy*, 69(9), e00320-25 (2025), PubMed: 40757818 2024 The bacterial division protein MinDE has an independent function in flagellation Pradhan P, Taviti AC, Beuria TK, *Journal of Biological Chemistry*, 300(4), 107117 (2024), PubMed: 38403244 2023 The cell division protein ZapE is targeted by the antibiotic aztreonam to induce cell filamentation in Escherichia coli Guru A, Taviti AC, Sethy M, Ray S, Dixit A, Beuria TK, *FEBS Letters*, 597(23), 2931–2945 (2023), PubMed: 37857499 FtsE, the nucleotide binding domain of the ABC transporter homolog FtsEX, regulates septal PG synthesis in E. coli Mallik S, Dodia H, Ghosh A, Srinivasan R, Good L, Raghav SK, Beuria TK, *Microbiology Spectrum*, 11(3), e02863-22 (2023), PubMed: 37014250 2022 Long-read 16S-seq reveals nasopharynx microbial dysbiosis and enrichment of Mycobacterium and Mycoplasma in COVID-19 patients Prasad P, Mahapatra S, Mishra R, Murmu KC, et al. *Molecular Omics*, 18(6), 490–505 (2022), DOI: 10.1039/d2mo00044j Isolation and characterization of five SARS-CoV-2 strains of different clades and lineages circulating in Eastern India Singh B, Avula K, Chatterjee S, Datey A, et al. *Frontiers in Microbiology*, 13, 856913 (2022, DOI: 10.3389/fmicb.2022.856913 MBZM-N-IBT, a novel small molecule, restricts Chikungunya virus infection by targeting nsP2 protease activity in vitro, in vivo, and ex vivo De S, Ghosh S, Keshry SS, Mahish C, Mohapatra C, Guru A, et al. *Antimicrobial Agents and Chemotherapy*, 66(7), e00463-22 (2022), DOI: 10.1128/aac.00463-22 Kanamycin-Mediated Conformational Dynamics of E. coli outer membrane protein TolC Pattanayak BS, Dehury B, Priyadarshinee M, Jha S, Beuria TK, *Integrative Structural Biology of Proteins and Macromolecular Assemblies* (Book Chapter) (2022), Search on Google Scholar 2021 Targeting the achilles heel of FtsZ: the interdomain cleft Pradhan P, Margolin W, Beuria TK, *Frontiers in Microbiology*, 12, 732796 (2021), DOI: 10.3389/fmicb.2021.732796 Identification of multipotent drugs for COVID-19 therapeutics with the evaluation of their SARS-CoV-2 inhibitory activity Kumar S, Singh B, Kumari P, Kumar P, et al. *Computational and Structural Biotechnology Journal*, 19, 1998–2017 (2021), DOI: 10.1016/j.csbj.2021.04.014 Clinical, virological, immunological, and genomic characterization of asymptomatic and symptomatic cases with SARS-CoV-2 infection in India Chatterjee S, Datey A, Sengupta S, Ghosh A, et al. *Frontiers in Cellular and Infection Microbiology*, 11, 725035 (2021), DOI: 10.3389/fcimb.2021.725035 Biochemical characterization of an E. coli cell division factor FtsE shows ATPase cycles similar to the NBDs of ABC-transporters Mallick S, Kumar A, Dodia H, Alexander C, Vasudevan D, Beuria TK, *Bioscience Reports*, 41(1), BSR20203034 (2021), DOI: 10.1042/BSR20203034 Kanamycin-mediated conformational dynamics of Escherichia coli outer membrane protein Tol Pattanayak BS, Dehury B, Priyadarshinee M, et al. *Frontiers in Molecular Biosciences*, 8, 636286 (2021) DOI: 10.3389/fmolb.2021.636286 Comparative profile of ocular surface microbiome in vernal keratoconjunctivitis patients and healthy subjects Vishwakarma P, Mitra S, Beuria T, Barik MR, et al. *Graefe’s Archive for Clinical and Experimental Ophthalmology*, 259(7), 1925–1933 (2021), DOI: 10.1007/s00417-021-05109-z Nanopore 16S rRNA sequencing reveals alterations in nasopharyngeal microbiome and enrichment of Mycobacterium and Mycoplasma in patients with COVID-19 Mahapatra S, Mishra R, Prasad P, Murmu KC, et al. *medRxiv (Preprint)* (2021) DOI: 10.1101/2021.11.10.21266147 Isolation and Characterization of SARS-CoV-2 strains circulating in Eastern India Singh B, Avula K, Chatterjee S, Datey A, et al. *bioRxiv (Preprint)* (2021), DOI: 10.1101/2021.12.13.472526 2020 MazEF-rifampicin interaction suggests a mechanism for rifampicin induced inhibition of persisters Alexander C, Guru A, Pradhan P, Mallick S, et al. *BMC Molecular and Cell Biology*, 21(1), 73 (2020) DOI: 10.1186/s12860-020-00316-8 Analysis of Indian SARS-CoV-2 genomes reveals prevalence of D614G mutation in spike protein predicting an increase in interaction with TMPRSS2 and virus infectivity Raghav S, Ghosh A, Turuk J, Kumar S, et al. *Frontiers in Microbiology*, 11, 594928 (2020) DOI: 10.3389/fmicb.2020.594928 SARS-CoV-2 genome analysis of Indian isolates and molecular modelling of D614G mutated spike protein with TMPRSS2 Raghav S, Ghosh A, Turuk J, Kumar S, et al. *BioRxiv (Preprint)* (2020), DOI: 10.1101/2020.07.23.217430 Identification of drugs targeting multiple viral and human proteins using computational analysis for repurposing against COVID-19 Kumar S, Kumari P, Agnihotri G, Vijay A, et al. *Preprint* (2020), https://doi.org/10.26434/chemrxiv.12366938.v1 2019 Bacterial Min proteins beyond the cell division Taviti AC, Beuria TK, *Critical Reviews in Microbiology*, 45(1), 22–32 (2019) DOI: 10.1080/1040841X.2018.1538932 2017 MinD directly interacting with FtsZ at the H10 helix suggests a model for robust activation of MinC to destabilize FtsZ polymers Taviti AC, Beuria TK, *Biochemical Journal*, 474(18), 3189–3205 (2017), DOI: 10.1042/BCJ20170357 Green synthesis of multi-metallic nanocubes Patra N, Taviti AC, Sahoo A, Pal A, et al. *RSC Advances*, 7(56), 35111–35118 (2017) https://doi.org/10.1039/C7RA05493A Y-shaped morphology in E. coli may be linked to peptidoglycan synthesis pathway Mallick S, Beuria TK, *Canadian Journal of Biotechnology*, 1(Special), 160 (2017) https://www.proquest.com/openview/8c2c70d71edaf70fc26a078319c452b4/1?pq-origsite=gscholar&cbl=4385532 2015 Doxorubicin inhibits E. coli division by interacting at a novel site in FtsZ Panda P, Taviti AC, Satpati S, Kar MM, Dixit A, Beuria TK, *Biochemical Journal*, 471(3), 335–346 (2015) DOI: 10.1042/BJ20150467 Synthesis, characterisation and antibacterial activity of [(p-cym)RuX(L)]+/2+ complexes Tripathy SK, Taviti AC, Dehury N, Sahoo A, Pal S, Beuria TK, et al. *Dalton Transactions* (2015) https://doi.org/10.1039/C4DT03647F 2013 In vitro evaluation of anti-infective activity of a Lactobacillus plantarum strain against Salmonella enterica serovar Enteritidis Das JK, Mishra D, Ray P, Tripathy P, et al. *Gut Pathogens*, 5(1), 11 (2013), DOI: 10.1186/1757-4749-5-11 2010 Molecular mechanism by which the nucleoid occlusion factor, SlmA, keeps cytokinesis in check Tonthat NK, Arold ST, Pickering BF, Van Dyke MW, Liang S, Lu Y, Beuria TK, Margolin W, Schumacher MA *The EMBO Journal*, 30(1), 154 (2010), DOI: 10.1038/emboj.2010.285 Bacterial cytokinesis: FzlA frizzes FtsZ filaments for fission force Beuria TK, Margolin W. *Current Biology*, 20(23), R1024–R1027 (2010), DOI: 10.1016/j.cub.2010.10.024 2009 Promoting assembly and bundling of FtsZ as a strategy to inhibit bacterial cell division: a new approach for developing novel antibacterial drugs Beuria TK, Singh P, Surolia A, Panda D, *Biochemical Journal*, 423(1), 61–69 (2009), DOI: 10.1042/BJ20090817 Adenine nucleotide-dependent regulation of assembly of bacterial tubulin-like FtsZ by a hypermorph of bacterial actin-like FtsA Beuria TK, Mullapudi S, Mileykovskaya E, et al. *Journal of Biological Chemistry*, 284(21), 14079–14086 (2009), DOI: 10.1074/jbc.M808872200 2007 Totarol inhibits bacterial cytokinesis by perturbing the assembly dynamics of FtsZ Jaiswal R, Beuria TK, Mohan R, Mahajan SK, Panda D. *Biochemistry*, 46(14), 4211–4220 (2007) https://doi.org/10.1021/bi602573e Interaction between cell division proteins FtsE and FtsZ Corbin BD, Wang Y, Beuria TK, Margolin W, *Journal of Bacteriology*, 189(8), 3026–3035 (2007) DOI: 10.1128/JB.01839-06 2006 Effects of pH and ionic strength on the assembly and bundling of FtsZ protofilaments: a possible role of electrostatic interactions in the bundling of protofilaments Beuria TK, Shah JH, Santra MK, Kumar V, et al. *International Journal of Biological Macromolecules*, 40(1), 30–39 (2006), DOI: 10.1016/j.ijbiomac.2006.05.006 2005 Sanguinarine blocks cytokinesis in bacteria by inhibiting FtsZ assembly and bundling Beuria TK, Santra MK, Panda D. *Biochemistry*, 44(50), 16584–16593 (2005), https://doi.org/10.1021/bi050767+ A natural osmolyte trimethylamine N-oxide promotes assembly and bundling of the bacterial cell division protein, FtsZ and counteracts the denaturing effects of urea Mukherjee A, Santra MK, Beuria TK, Panda D. *The FEBS Journal*, 272(11), 2760–2772 (2005) DOI: 10.1111/j.1742-4658.2005.04696.x 2004 Ruthenium red-induced bundling of bacterial cell division protein, FtsZ Santra MK, Beuria TK, Banerjee A, Panda D. *Journal of Biological Chemistry*, 279(25), 25959–25965 (2004) https://doi.org/10.1074/jbc.m312473200 2003 Glutamate-induced assembly of bacterial cell division protein FtsZ Beuria TK, Krishnakumar SS, Sahar S, Singh N, Gupta K, Meshram M, Panda D. *Journal of Biological Chemistry*, 278(6), 3735–3741 (2003), DOI: 10.1074/jbc.M205698200

Details

2026

Efflux-Mediated Resistance in Staphylococcus aureus: Challenges and Opportunities for Therapeutic Intervention

Ojha S, Beuria TK, Indian Journal of Microbiology, 1–30 (2026), DOI: 10.1007/s12088-026-01545-9

2025

Efflux pump modulation by Montelukast and its roles in restoring antibiotic susceptibility in multidrug-resistant Staphylococcus aureus

Ojha S, Sinsinwar S, Chatterjee P, Biswal S, Pradhan P, Beuria TK, EBioMedicine, 114, 105658 (2025) PubMed: 40157128

10058-F4 Mediated inhibition of the biofilm formation in multidrug-resistant Staphylococcus aureus

Dodia H, Ojha S, Chatterjee P, Beuria TK, Biofilm, 8, 100307 (2025), DOI: 10.1016/j.bioflm.2025.100307

Inhibition of efflux pumps by FDA-approved drugs oxiconazole and sertaconazole restores antibiotic susceptibility in multidrug-resistant S. aureus

Ojha S, Chatterjee P, Beuria TK, Antimicrobial Agents and Chemotherapy, 69(9), e00320-25 (2025), PubMed: 40757818

2024

The bacterial division protein MinDE has an independent function in flagellation

Pradhan P, Taviti AC, Beuria TK, Journal of Biological Chemistry, 300(4), 107117 (2024), PubMed: 38403244

2023

The cell division protein ZapE is targeted by the antibiotic aztreonam to induce cell filamentation in Escherichia coli

Guru A, Taviti AC, Sethy M, Ray S, Dixit A, Beuria TK, FEBS Letters, 597(23), 2931–2945 (2023), PubMed: 37857499

FtsE, the nucleotide binding domain of the ABC transporter homolog FtsEX, regulates septal PG synthesis in E. coli

Mallik S, Dodia H, Ghosh A, Srinivasan R, Good L, Raghav SK, Beuria TK, Microbiology Spectrum, 11(3), e02863-22 (2023), PubMed: 37014250

2022

Long-read 16S-seq reveals nasopharynx microbial dysbiosis and enrichment of Mycobacterium and Mycoplasma in COVID-19 patients

Prasad P, Mahapatra S, Mishra R, Murmu KC, et al. Molecular Omics, 18(6), 490–505 (2022), DOI: 10.1039/d2mo00044j

Isolation and characterization of five SARS-CoV-2 strains of different clades and lineages circulating in Eastern India

Singh B, Avula K, Chatterjee S, Datey A, et al. Frontiers in Microbiology, 13, 856913 (2022,

DOI: 10.3389/fmicb.2022.856913

MBZM-N-IBT, a novel small molecule, restricts Chikungunya virus infection by targeting nsP2 protease activity in vitro, in vivo, and ex vivo

De S, Ghosh S, Keshry SS, Mahish C, Mohapatra C, Guru A, et al. Antimicrobial Agents and Chemotherapy, 66(7), e00463-22 (2022), DOI: 10.1128/aac.00463-22

Kanamycin-Mediated Conformational Dynamics of E. coli outer membrane protein TolC

Pattanayak BS, Dehury B, Priyadarshinee M, Jha S, Beuria TK, Integrative Structural Biology of Proteins and Macromolecular Assemblies (Book Chapter) (2022), Search on Google Scholar

2021

Targeting the achilles heel of FtsZ: the interdomain cleft

Pradhan P, Margolin W, Beuria TK, Frontiers in Microbiology, 12, 732796 (2021), DOI: 10.3389/fmicb.2021.732796

Identification of multipotent drugs for COVID-19 therapeutics with the evaluation of their SARS-CoV-2 inhibitory activity

Kumar S, Singh B, Kumari P, Kumar P, et al. Computational and Structural Biotechnology Journal, 19, 1998–2017 (2021), DOI: 10.1016/j.csbj.2021.04.014

Clinical, virological, immunological, and genomic characterization of asymptomatic and symptomatic cases with SARS-CoV-2 infection in India

Chatterjee S, Datey A, Sengupta S, Ghosh A, et al. Frontiers in Cellular and Infection Microbiology, 11, 725035 (2021), DOI: 10.3389/fcimb.2021.725035

Biochemical characterization of an E. coli cell division factor FtsE shows ATPase cycles similar to the NBDs of ABC-transporters

Mallick S, Kumar A, Dodia H, Alexander C, Vasudevan D, Beuria TK, Bioscience Reports, 41(1), BSR20203034 (2021), DOI: 10.1042/BSR20203034

Kanamycin-mediated conformational dynamics of Escherichia coli outer membrane protein Tol

Pattanayak BS, Dehury B, Priyadarshinee M, et al. Frontiers in Molecular Biosciences, 8, 636286 (2021)

DOI: 10.3389/fmolb.2021.636286

Comparative profile of ocular surface microbiome in vernal keratoconjunctivitis patients and healthy subjects

Vishwakarma P, Mitra S, Beuria T, Barik MR, et al. Graefe’s Archive for Clinical and Experimental Ophthalmology, 259(7), 1925–1933 (2021), DOI: 10.1007/s00417-021-05109-z

Nanopore 16S rRNA sequencing reveals alterations in nasopharyngeal microbiome and enrichment of Mycobacterium and Mycoplasma in patients with COVID-19

Mahapatra S, Mishra R, Prasad P, Murmu KC, et al. medRxiv (Preprint) (2021)

DOI: 10.1101/2021.11.10.21266147

Isolation and Characterization of SARS-CoV-2 strains circulating in Eastern India

Singh B, Avula K, Chatterjee S, Datey A, et al. bioRxiv (Preprint) (2021), DOI: 10.1101/2021.12.13.472526

2020

MazEF-rifampicin interaction suggests a mechanism for rifampicin induced inhibition of persisters

Alexander C, Guru A, Pradhan P, Mallick S, et al. BMC Molecular and Cell Biology, 21(1), 73 (2020)

DOI: 10.1186/s12860-020-00316-8

Analysis of Indian SARS-CoV-2 genomes reveals prevalence of D614G mutation in spike protein predicting an increase in interaction with TMPRSS2 and virus infectivity

Raghav S, Ghosh A, Turuk J, Kumar S, et al. Frontiers in Microbiology, 11, 594928 (2020)

DOI: 10.3389/fmicb.2020.594928

SARS-CoV-2 genome analysis of Indian isolates and molecular modelling of D614G mutated spike protein with TMPRSS2

Raghav S, Ghosh A, Turuk J, Kumar S, et al. BioRxiv (Preprint) (2020), DOI: 10.1101/2020.07.23.217430

Identification of drugs targeting multiple viral and human proteins using computational analysis for repurposing against COVID-19

Kumar S, Kumari P, Agnihotri G, Vijay A, et al. Preprint (2020), https://doi.org/10.26434/chemrxiv.12366938.v1

2019

Bacterial Min proteins beyond the cell division

Taviti AC, Beuria TK, Critical Reviews in Microbiology, 45(1), 22–32 (2019)

DOI: 10.1080/1040841X.2018.1538932

2017

MinD directly interacting with FtsZ at the H10 helix suggests a model for robust activation of MinC to destabilize FtsZ polymers

Taviti AC, Beuria TK, Biochemical Journal, 474(18), 3189–3205 (2017), DOI: 10.1042/BCJ20170357

Green synthesis of multi-metallic nanocubes

Patra N, Taviti AC, Sahoo A, Pal A, et al. RSC Advances, 7(56), 35111–35118 (2017)

https://doi.org/10.1039/C7RA05493A

Y-shaped morphology in E. coli may be linked to peptidoglycan synthesis pathway

Mallick S, Beuria TK, Canadian Journal of Biotechnology, 1(Special), 160 (2017)

https://www.proquest.com/openview/8c2c70d71edaf70fc26a078319c452b4/1?pq-origsite=gscholar&cbl=4385532

2015

Doxorubicin inhibits E. coli division by interacting at a novel site in FtsZ

Panda P, Taviti AC, Satpati S, Kar MM, Dixit A, Beuria TK, Biochemical Journal, 471(3), 335–346 (2015)

DOI: 10.1042/BJ20150467

Synthesis, characterisation and antibacterial activity of [(p-cym)RuX(L)]+/2+ complexes

Tripathy SK, Taviti AC, Dehury N, Sahoo A, Pal S, Beuria TK, et al. Dalton Transactions (2015)

https://doi.org/10.1039/C4DT03647F

2013

In vitro evaluation of anti-infective activity of a Lactobacillus plantarum strain against Salmonella enterica serovar Enteritidis

Das JK, Mishra D, Ray P, Tripathy P, et al. Gut Pathogens, 5(1), 11 (2013), DOI: 10.1186/1757-4749-5-11

2010

Molecular mechanism by which the nucleoid occlusion factor, SlmA, keeps cytokinesis in check

Tonthat NK, Arold ST, Pickering BF, Van Dyke MW, Liang S, Lu Y, Beuria TK, Margolin W, Schumacher MA

The EMBO Journal, 30(1), 154 (2010), DOI: 10.1038/emboj.2010.285

Bacterial cytokinesis: FzlA frizzes FtsZ filaments for fission force

Beuria TK, Margolin W. Current Biology, 20(23), R1024–R1027 (2010), DOI: 10.1016/j.cub.2010.10.024

2009

Promoting assembly and bundling of FtsZ as a strategy to inhibit bacterial cell division: a new approach for developing novel antibacterial drugs

Beuria TK, Singh P, Surolia A, Panda D, Biochemical Journal, 423(1), 61–69 (2009), DOI: 10.1042/BJ20090817

Adenine nucleotide-dependent regulation of assembly of bacterial tubulin-like FtsZ by a hypermorph of bacterial actin-like FtsA

Beuria TK, Mullapudi S, Mileykovskaya E, et al. Journal of Biological Chemistry, 284(21), 14079–14086 (2009), DOI: 10.1074/jbc.M808872200

2007

Totarol inhibits bacterial cytokinesis by perturbing the assembly dynamics of FtsZ

Jaiswal R, Beuria TK, Mohan R, Mahajan SK, Panda D. Biochemistry, 46(14), 4211–4220 (2007)

https://doi.org/10.1021/bi602573e

Interaction between cell division proteins FtsE and FtsZ

Corbin BD, Wang Y, Beuria TK, Margolin W, Journal of Bacteriology, 189(8), 3026–3035 (2007)

DOI: 10.1128/JB.01839-06

2006

Effects of pH and ionic strength on the assembly and bundling of FtsZ protofilaments: a possible role of electrostatic interactions in the bundling of protofilaments

Beuria TK, Shah JH, Santra MK, Kumar V, et al. International Journal of Biological Macromolecules, 40(1), 30–39 (2006), DOI: 10.1016/j.ijbiomac.2006.05.006

2005

Sanguinarine blocks cytokinesis in bacteria by inhibiting FtsZ assembly and bundling

Beuria TK, Santra MK, Panda D. Biochemistry, 44(50), 16584–16593 (2005), https://doi.org/10.1021/bi050767+

A natural osmolyte trimethylamine N-oxide promotes assembly and bundling of the bacterial cell division protein, FtsZ and counteracts the denaturing effects of urea

Mukherjee A, Santra MK, Beuria TK, Panda D. The FEBS Journal, 272(11), 2760–2772 (2005)

DOI: 10.1111/j.1742-4658.2005.04696.x

2004

Ruthenium red-induced bundling of bacterial cell division protein, FtsZ

Santra MK, Beuria TK, Banerjee A, Panda D. Journal of Biological Chemistry, 279(25), 25959–25965 (2004)

https://doi.org/10.1074/jbc.m312473200

2003

Glutamate-induced assembly of bacterial cell division protein FtsZ

Beuria TK, Krishnakumar SS, Sahar S, Singh N, Gupta K, Meshram M, Panda D. Journal of Biological Chemistry, 278(6), 3735–3741 (2003), DOI: 10.1074/jbc.M205698200

Group

Details

Current Members

Dr. Sourav Das	DST – Inspire Faculty	Project: Studying the Ecological Dynamics of Microbial Predator-Prey Interactions in Soil for Agricultural Benefits
Dr. Sarmistha Biswal	Project Scientist-I	Project: Studying the industrially important enzymes, biomolecules & secondary metabolites
Dr. Suvendu Ojha	Research Associate-I	Project: Pre-Clinical Validation of Montelukast-Moxifloxacin Combination Therapy for Multidrug-Resistant Staphylococcus aureus Sepsis
Hiren G. Dodia	Senior Research Fellow (Ph.D.)	Project: Targeting Bacterial Division and Biofilm Formation to Combat Bacterial Resilience
Srusti Ray	Senior Research Fellow (Ph.D.)	Project: Biochemical characterization of the drug binding sites in FtsZ and targeting it for the identification of new antibacterial molecules
Rakesh Mohapatra	Senior Research Fellow (Ph.D.)	Project: Investigating the role of Toxin-Antitoxin (TA) modules in bacterial persistence phenotype and other physiological activity
Puja Chatterjee	Senior Research Fellow (Ph.D.)	Project: Efflux Pumps Contribution To Biofilm-Mediated Antibiotic Resistance And Development of Dual Inhibitors Against Multidrug-Resistant Staphylococcus aureus
Anidrisha Sahu	Junior Research Fellow (Ph.D.)	Project: To be decided.
Itipragyan Singh	Junior Research Fellow (Ph.D.)	Project: Mechanistic Insights into Efflux Pump Functionality for Restoring Antibiotic Sensitivity in Multi-Drug Resistant Bacteria
Yash Tatwade	Junior Research Fellow (Ph.D.)	Project: Development of membrane-active peptides as antibacterials to target efflux pumps and biofilm formation to tackle bacterial resistance
Mitali Madhusmita Kar	Lab Technician	Project: –
Niharika Singh	Project Technician	Project: Exploring the marine ecosystems for novel bioresources with human health and industrial significance
Megha Seal	Project Associate-I	Project: Establishment of OMBRIC-ILS Centre of Excellence in Marine Biotechnology for promoting research on bioprospecting of marine biota
Dushmanta Parida	Project Technician	Project: Studying the Ecological Dynamics of Microbial Predator-Prey Interactions in Soil for Agricultural Benefits

Our Graduates

	Dr. Pinkilata Pradhan (Ph.D.)	Postdoc at Umea, Sweden
	Dr. Ankeeta Guru (Ph.D.)	Postdoc at Department of Health, Wardsworth Center, New York, USA
	Dr. Cyrus Alexander (Ph.D.)	Postdoc at UMass Chan Medical School, USA
	Dr. Sunanda Mallick (Ph.D.)	Postdoc at Yale School of Medicine, USA
	Dr. T. Ashoka Chary (Ph.D.)

Lab Alumni

	Ms. Deepika Jena (Junior Research Fellow)
	Anjali Sharma (Junior Research Fellow)
	Dr. Pragnya Panda (DBT-Research Associate)
	Dr. Simran Sinsinwar (Research Associate-I)
	Dr. Sudhakar Pagal (Project Scientist-I)

Grants

Details

Extramural Grants:

Title of the Project	Funding Agency	Grant Period
Screening and identification of efflux pump inhibitors to combat antibacterial resistance in multidrug-resistant S. aureus.	DST	2022-2025
To understand the role of FtsEX in coordinating the cell division with the membrane cleavage in E. coli.	DBT	2018 – 2021
To understand the oscillation mechanism of the Min system and its role in coordinating cell division with cell motility.	DST	2018 – 2021
Design, synthesis, and biological evaluation of novel FtsZ inhibitors as potential anti-tubercular agents	DBT	2013 -2016

Contacts

Email	Address	Fax	Office
tkbeuria@ils.res.in	BRIC-Institute of Life Sciences, Nalco Square, Bhubaneswar-751023, India	0091 674 2300728	0091 674 2302640

Highlights

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Publications

Lab Group 2026

Positions

Details
PhD Opportunities: I am always looking for motivated and intellectually curious candidates with a valid CSIR–UGC JRF or equivalent national fellowship to join my research group. Candidates with a strong interest in pursuing rigorous, hypothesis-driven research are encouraged to get in touch. Postdoctoral Fellow / Research Associate / N-PDF: I welcome applications from candidates who have recently submitted their PhD thesis and are eager to engage in advanced research. If you are driven, independent, and interested in contributing to ongoing projects in my lab, please reach out with your updated CV. Enthusiastic candidates with a strong research mindset are particularly encouraged to contact me.

Details

PhD Opportunities:

I am always looking for motivated and intellectually curious candidates with a valid CSIR–UGC JRF or equivalent national fellowship to join my research group. Candidates with a strong interest in pursuing rigorous, hypothesis-driven research are encouraged to get in touch.

Postdoctoral Fellow / Research Associate / N-PDF:

I welcome applications from candidates who have recently submitted their PhD thesis and are eager to engage in advanced research. If you are driven, independent, and interested in contributing to ongoing projects in my lab, please reach out with your updated CV.

Enthusiastic candidates with a strong research mindset are particularly encouraged to contact me.

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