Awards:

• Prof. KS Bilgrami Memorial Award-2018 from Society for Plant Research, India.
• E. K. Janaki National Award (2015) from Union Minister for Environment, Forest and climate change, Govt. of India.
• Outstanding contribution award (2014) from Association for the Advancement of Biodiversity Science, Karnataka, India.

Recognitions:

• Honorary Life Membership, Society for Plant Research, India
• Member, American Society of Microbiology, USA
• Elected Fellow, West Bengal Academy of Science and Technology, West Bengal, India
• Elected Fellow, Association for the Advancement of Biodiversity Science, Karnataka, India
• Invited Faculty for the International Thematic School on Aquatic Microbial Ecology (ASIAME 2) for South Asia on “Marine Microbial Ecology” as context of a French-Indian cooperation

Microbial Genomics, Gene Function and Regulation

Research Interest

My laboratory is working on microbial genomics towards translational research. In this regard, useful microbes with biotechnological potential have been characterized from hot springs and marine environments for bioprospecting of novel genes and metabolic pathways. In this endeavour, we have characterized an environmental Bordetella isolate as a potential resource for the evolution of Bordetella bronchiseptica hybrid strain for the development of whole cell livestock vaccine. Further, we are using metagenomic approach to unveil the community composition and functional correlation of microbiome in pristine environment. Following polyphasic approach, 20 new microbes have been discovered from unmanaged ecosystem. Our future goal to address the genome scale reconstruction of metabolic model with these microbes for the functional analysis of genes involved in synthesis of metabolites, pharmaceuticals and ecological adaptation.

I. To study the mechanism of thiosulfate oxidation in an obligately mixotrophic bacterium Thiomonas bhubaneswarensis strain S10 (DSM 18181^T).

This study described the mechanism of electron transport during thiosulfate oxidation by T. bhubaneswarensis DSM 18181^T. Whole genome sequence analysis revealed the presence of complete sox (sulfur oxidation) gene cluster (soxCDYZAXB) including the sulfur oxygenase reductase (SOR), sulfide quinone reductase (SQR), sulfide dehydrogenase (fcc), thiosulfate dehydrogenase (Tsd), sulfite dehydrogenase (SorAB) and intracellular sulfur oxidation protein (DsrE/DsrF). In addition, genes encoding respiratory electron transport chain components viz. complex I (NADH dehydrogenase), complex II (succinate dehydrogenase), complex III (Ubiquinone-cytochrome c reductase) and various types of terminal oxidases (cytochrome c– and quinol- oxidase) were identified in the genome (Figure). Using site-specific electron donors, inhibitors, and by analyzing the cytochrome spectra, we identified the shortest thiosulfate dependent electron transport chain in T. bhubaneswarensis DSM 18181^T. Our results showed thiosulfate supports the electron transport activity in a bifurcated manner, donating electrons to quinol (bd)- and cytochrome c (Caa₃)- oxidase; these two sites (quinol oxidase and cytochrome c oxidase) also showed differences in their phosphate esterification potential (P/O). Further, it was evidenced that the substrate level phosphorylation is the major contributor to the total energy budget in this bacterium.

II. Functional characterization of microbiome and their correlation with physicochemical properties of geothermal springs by metagenomic approach

This study made significantly to understand the composition of microbiome in the geothermal springs and their correlation with the physicochemical properties of the hot springs by using the metagenomic approach. Metagenome sequence data showed a dominance of Bacteria over Archaea; the most abundant phyla were Chloroflexi and Proteobacteria. The distribution of major genera and their statistical correlation analyses with the physicochemical parameters predicted that the temperature, aqueous concentrations of ions (such as sodium, chloride, sulfate, and bicarbonate), total hardness, dissolved solids and conductivity were the main environmental variables influencing microbial community composition and diversity (Figure). In conclusion, we have reported the phylogenetic and functional diversity of microbial communities in the hot springs.

III. Genomic characterization of two novel chromosomally integrating conjugative elements ICEMfuInd1a and ICEMfuInd1b from a marine bacterium Marinomonas fungiae JCM 18476^T.

The coexistence of two Integrating conjugative elements (ICEs) of the SXT exclusion group in the same genome is very rare and limited information is available on natural isolates harbouring such SXT/R391 ICEs arrays. Our studies with Marinomonas fungiae JCM 18476^T has identified the coexistence of two new SXT/R391-like ICEs (ICEMfuInd1a and ICEMfuInd1b) in the genome.  Phylogenetic analysis revealed the two ICEs either evolved independently or high degrees of recombination events have masked their common evolution from SXT-like ancestors. Further, we found that the typical entry exclusion mechanism mediated by the TraG/EeX protein pair was likely defective in preventing the conjugative transfer of a second copy of the same S (SXT) group ICE in M. fungiae due to mutations. Our analysis showed the presence of 16 and 25 variable genes in the hotspots of ICEMfuInd1a and ICEMfuInd1b respectively, many of which were not reported earlier for SXT/R391 ICEs (Figure). Sequence analysis predicted these hotspot regions were shaped by acquisition of genes through homologous recombination between the SXT and R391 related ICEs or mobile genetic elements present in disparate marine bacteria.

Microbial Ecology, Genomics and Translational Research

Publications  

1. Manish Prakash Victor, Ritu Rani Archana Kujur, Lipika Das and Subrata K. Das^*. 2024. Genome analysis deciphered Chryseobacterium indicum is a distinct species associated with freshwater pufferfish. Infection, Genetics and Evolution. 118: 105561. https://doi.org/10.1016/j.meegid.2024.105561. IF-4.4
2. Jhasketan Badhai and Subrata K. Das*. 2023. Molecular basis of origin, virulence properties and pathogenicity of obligate host-restricted and the environmental strains of Bordetella. Journal of Applied Microbiology. 134: lxad200. doi: 10.1093/jambio/lxad200. IF-4.0
3. Lipika Das, Ritu Rani Archana Kujur, Tanmoy Debnath and Subrata K Das^*. 2023.  Phylogeny and genomic analysis of Shewanella cutis sp. nov., isolated from freshwater pufferfish. Folia Microbiologica (Praha). 24: doi: 10.1007/s12223-023-01111-6. IF-2.7
4. Ritu Rani Archana Kujur, Manisha Ghosh, Surajit Basak and Subrata K Das^*. 2023. Phylogeny and structural insights of lipase from Halopseudomonas maritima sp. nov., isolated from sea sand. International Microbiology. https://doi.org/10.1007/s10123-023-00362-0. IF-3.2
5. Tanmoy Debnath, Sushanta Deb and Subrata K Das^*. 2023. Influence of Geochemistry in the Tropical Hot Springs on Microbial Community Structure and Function. Current Microbiology. 80: 4. https://doi.org/10.1007/s00284-022-03118-7
6. Sushanta Deb and Subrata K Das^*. 2022. Phylogenomic Analysis of Metagenome-Assembled Genomes Deciphered Novel Acetogenic Nitrogen-Fixing Bathyarchaeota from Hot Spring Sediments. Microbiology Spectrum. 10: e00352-22. (ASM, USA). doi.org/10.1128/spectrum.00352-22.
7. Lipika Das and Subrata K Das*. 2022. Moraxella tetraodonis sp. nov., isolated from freshwater pufferfish (Tetraodon cutcutia) skin. Archives of Microbiology. 204:389. https://doi.org/10.1007/s00203-022-03013-6. IF-2.55
8. Ritu Rani Archana Kujur and Subrata K Das^*. 2022. Pseudomonas phenolilytica sp. nov., a novel phenol degrading bacterium. Archives of Microbiology. 204: 320. https://doi.org/10.1007/s00203-022-02912-y.
9. Lipika Das, Sushanta Deb and Subrata K. Das*. 2022. Microbial community composition in the skin of pufferfish (Tetraodon cutcutia) Isolated from Mahanadi River. Green Technologies for Environmental Management. Book Chapter-18. Astral International Pvt. Ltd. Pp. 229-236.
10. Ritu Rani Archana Kujur, Sushanta Deb and Subrata K Das^*. 2022. Genome analysis of Pseudomonas species reveals that Pseudomonas panacis Park et al. 2005 is a later heterotypic synonym of Pseudomonas marginalis (Brown 1918) Stevens 1925 (Approved Lists 1980). Int. J. Syst. Evol. Microbiol.  72: 005354. DOI 10.1099/ijsem.0.005354.
11. Tanmoy Debnath and Subrata K Das^*. 2022. Draft genome sequence of Plesiomonas shigelloides strain PI-19, isolated from the intestine of pufferfish (Tetraodon cutcutia). Microbiology Resource Announcements. 11: e0125221.
12. Lipika Das, Sushanta Deb, Eiji Arakawa, Shinji Yamasaki, and Subrata K. Das^*. 2022.Pufferfish (Tetraodon cutcutia) Sampled from a Freshwater River Serves as an Intermediate Reservoir of a Sucrose Nonfermenting Variant of Vibrio cholerae PS-4. Microbiology Spectrum. 10: e01221-21.
13. Lipika Das, Sushanta Deb and Subrata K. Das^*. 2021. Description of Acinetobacter kanungonis sp. nov., based on phylogenomic analysis. Int. J. Syst. Evol. Microbiol. DOI:10.1099/ijsem.0.004833
14. Jhasketan Badhai and Subrata K. Das*. 2021. Genomic plasticity and antibody response of Bordetella bronchiseptica strain HT200, a natural variant from a thermal spring. FEMS Microbiology Letters.  368(6): fnab035. (Oxford Univ. Press). doi.org/10.1093/femsle/fnab035.
15. Prabla Kumari, Abhijit Poddar and Subrata K. Das^*. 2020. Characterization of multidrug resistance in Vibrio species isolated from marine invertebrates from Andaman Sea. 3Biotech. 10: 456. DOI: 10.1007/s13205-020-02445-5
16. Sushanta Deb, Lipika Das and Subrata K. Das^*. 2020. Composition and functional characterization of the gut microbiome of fresh water puffer fish (Tetraodon cutcutia). Archives Microbiology. 202: 2761-2770. DOI: 10.1007/s00203-020-01997-7.
17. Sushanta Deb, Lipika Das and Subrata K. Das^*. 2020. Phylogenomic analysis reveals that Arthrobacter mysorens Nand and Rao 1972 (Approved Lists 1980) and Glutamicibacter mysorens Busse 2016 are later heterotypic synonyms of Arthrobacter nicotianae Giovannozzi-Sermanni 1959 (Approved Lists 1980) and Glutamicibacter nicotianae Busse 2016. Current Microbiology. 77: 3793-3798. DOI: 10.1007/s00284-020-02176-z.
18. Lipika Das, Sushanta Deb and Subrata K. Das^*. 2020. Glutamicibacter mishrai sp. nov., isolated from the coral Favia veroni from Andaman Sea. Archives Microbiology. 202: 733-745. doi 10.1007/s00203-019-01783-0. 
19. Sushanta Deb, Jhasketan Badhai and Subrata K. Das*. 2020. Draft genome sequence of Vibrio alginolyticus strain S6-61 and Vibrio diabolicus strain S7-71 isolated from corals from Andaman Sea. Microbiology Resource Announcements. 9(8):    e01465-19. https://doi.org/10.1128/MRA.01465-19.
20. Sushanta Deb and Subrata K. Das*. 2020. Draft Genome Sequence of Microbacterium oryzae strain MB-10 isolated from a rice field, India. Microbiology Resource Announcements. 9(6): e01532-19. https://doi.org/10.1128/MRA.01532-19.
21. Sushanta Deb and Subrata K. Das*. 2020. Draft Genome sequence of Idiomarina strain W5T isolated from Andaman Sea. Microbiology Resource Announcements. 9(5): e01510-19. https://doi.org/10.1128/MRA.01510-19.
22. Sushanta Deb, Jhasketan Badhai and Subrata K. Das*. 2020. Draft genome sequence of two Vibrio fortis strains isolated from coral Fungia from Andaman Sea. Microbiology Resource Announcements. 9(1): e01225-19. https://doi.org/10.1128/MRA.01225-19.
23. Romit Mitra, Lipika Das and Subrata K. Das*. 2019. Gut Microbiome and Their Impact on Human Health.Vol:1. Microbial Diversity in Normal & Extreme Environments. Springer Nature Singapore Pte Ltd. 355-385. DOI: https://doi.org/10.1007/978-981-13-8315-1_12.
24. Tanmoy Debnath, Ritu Rani Archana Kujur, Romit Mitra and Subrata K. Das*. 2019.Diversity of Microbes in Hot Springs and Their Sustainable Use.Vol:1. Microbial Diversity in Normal & Extreme Environments. Springer Nature Singapore Pte Ltd. 159-186. DOI: https://doi.org/10.1007/978-981-13-8315-1_6.
25. Jhasketan Badhai, Sushanta Deb and Subrata K. Das*. 2019. Application of Genomics to Understand the Pathogenic Microbial Diversity. Vol:1. Microbial Diversity in Normal & Extreme Environments. Springer Nature Singapore Pte Ltd.Pp- 49-92. DOI: https://doi.org/10.1007/978-981-13-8315-1_3.
26. T. Satyanarayana, BN Johriand Subrata K Das (Editors).2019.Microbial Diversity in Ecosystem Sustainability and Biotechnological Applications. Vol:1. Microbial Diversity in Normal & Extreme Environments. Springer Nature Singapore Pte Ltd. DOI: https://doi.org/10.1007/978-981-13-8315-1.
27. T. Satyanarayana, Subrata K Das and BN Johri (Editors).2019.Microbial Diversity in Ecosystem Sustainability and Biotechnological Applications. Vol: 2. Soil & Agroecosystems. Springer Nature Singapore Pte Ltd. DOI: https://doi.org/10.1007/978-981-13-8487-5.
28. A.T. Hinsu, R.J. Pandit, S.H. Patela, A. Psifidic, F.M Tomley, Subrata K. Das, Damer P. Blake, C.G. Joshi. 2019. Genome reconstruction of a novel carbohydrate digesting bacterium from the chicken caecal microflora. Meta Gene. 20: 100543. doi.org/10.1016/j.mgene.2019.01.002.
29. Prabla Kumari, Jhasketan Badhai and Subrata K. Das^*. 2018. Draft genome sequence of Marinomonas fungiae strain AN44^T (JCM 18476^T) isolated from the coral Fungia echinata from Andaman Sea. Genome Announcements. 6(10): e00112-18.
30. Abhijit Poddar and Subrata K Das^*. 2018. Microbiological studies of hot springs in India. A Review. Archives of Microbiology. 200: 1-18. DOI: 10.1007/s00203-017-1429-3.
31. Sherin Zachariah and Subrata K Das^*. 2017. Idiomarina andamanensis sp. nov., an alkalitolerant bacterium isolated from Andaman Sea water. Antonie van Leeuwenhoek. 110: 1581–1592. DOI: 10.1007/s10482-017-0908-5
32. Jhasketan Badhai, William B. Whitman and Subrata K. Das^*. 2017. Draft genome sequence of Rhizobium pusense strain NRCPB10^T (LMG 25623^T) isolated from rhizosphere soil of chickpea (Cicer arietinum L.) grown in India. Genome Announcements. 5(17): e00282-17. DOI: 10.1128/genomeA.0028217.
33. Saumya Bandyopadhyay, William B. Whitman and Subrata K Das^*. 2017. Draft genome sequence of Pannonibacter indicus strain HT23^T (DSM 23407^T), a highly arsenate tolerant bacterium isolated from a hot spring in India. Genome Announcements. 5(18): e00283-17. DOI: 10.1128/genomeA.0028317.   
34. Sherin Zachariah, Prabla Kumari, and Subrata K Das^*. 2017. Sulfitobacter pontiacus subsp. fungiae subsp. nov., isolated from coral Fungia seychellensis from Andaman Sea, and description of Sulfitobacter pontiacus subsp. pontiacus subsp. nov. Current Microbiology, 74: 404-412. doi: 10.1007/s00284-017-1200-7.
35. Kunwar Digvijay Narayan, Surendra Chandra Sabat and Subrata K Das^*. 2017. Mechanism of electron transport during thiosulfate oxidation in an obligately mixotrophic bacterium Thiomonas bhubaneswarensis strain S10 (DSM 18181^T). Applied Microbiology and Biotechnology,  101: 1239-1252. doi: 10.1007/s00253-016-7958-x.
36. Jhasketan Badhai and Subrata K Das^*. 2016. Characterization of three novel SXT/R391 integrating conjugative elements ICEMfuInd1a and ICEMfuInd1b, and ICEMprChn1 identified in the genomes of Marinomonas fungiae JCM 18476^T and Marinomonas profundimaris strain D104. Frontiers in Microbiology, 7: 1896. doi: 10.3389/fmicb.2016.01896.
37. Sherin Zachariah, Prabla Kumari and Subrata K Das^*. 2016. Psychrobacter pocilloporae sp. nov., isolated from coral Pocillopora eydouxi from Andaman Sea. International Journal of Systematic and Evolutionary Microbiology, 66: 5091-5098. doi: 10.1099/ijsem.0.001476.
38. Jhasketan Badhai, Tarini Shankar Ghosh and Subrata K Das^*. 2016. Composition and functional characterization of microbiome associated with mucus of the coral Fungia echinata collected from Andaman Sea. Frontiers in Microbiology, 7: 936. doi: 10.3389/fmicb.2016.00936.
39. Prabla Kumari, Sayantani Bhattacharjee, Abhijit Poddar and Subrata K Das^*. 2016. Sulfitobacter faviae sp. nov., isolated from coral Favia veroni from Andaman Sea, India. International Journal of Systematic and Evolutionary Microbiology, 66: 3786-3792. doi: 10.1099/ijsem.0.001265.
40. Kunwar Digvijay Narayan, Jhasketan Badhai, William B. Whitman and Subrata K Das^*. 2016. Draft genome sequence of Comamonas thiooxydans strain S23^T (DSM 17888 ^T) a thiosulfate oxidizing bacterium isolated from a sulfur spring in India. Genome Announcements, 4(4): e00834-16. doi:10.1128/genomeA.00834-16.
    
41. Rinchen T Lepcha, Abhijit Poddar, William B. Whitman and Subrata K Das^*. 2016.  Draft genome sequence of Idiomarina woesei strain W11^T (DSM 27808^T) isolated from Andaman sea. Genome Announcements, 4(4): e00831-16. doi:10.1128/genomeA.00831-16.
42. Kamal Deep, Abhijit Poddar, William B. Whitman and Subrata K Das^*. 2016. Draft genome sequence of Anoxybacillus suryakundensis strain JS1^T (DSM 27374^T) isolated from hot spring at Jharkhand, India. Genome Announcements, 4(4): e00824-16. doi:10.1128/genomeA.00824-16.
    
43. Jhasketan Badhai, William B. Whitman and Subrata K Das^*. 2016. Draft genome sequence of Chelatococcus sambhunathii strain HT4^T (DSM18167^T) isolated from a hot spring in India. Genome Announcements, 4(4): e00825-16. doi:10.1128/genomeA.00825-16.
44. Jhasketan Badhai, Kunwar Digvijay Narayan, William B. Whitman and Subrata K Das^*. 2016. Draft genome sequence of Gulbenkiania indica strain HT27^T (DSM 17901^T) isolated from a sulfur spring in India. Genome Announcements, 4(4): e00830-16. doi:10.1128/genomeA.00830-16.
    
45. Abhijit Poddar, Rinchen T Lepcha, William B. Whitman and Subrata K Das.^* 2016. Draft genome sequence of Tepidiphilus thermophilus strain JHK30^T (JCM 19170^T) isolated from a terrestrial hot spring in India. Genome Announcements, 4(4): e00832-16. doi:10.1128/genomeA.00832-16.
46. Saumya Bandyopadhyay and Subrata K. Das^*. 2016. Functional analysis of ars gene cluster of Pannonibacter indicus strain HT23^T (DSM 23407^T) and identification of a proline residue essential for arsenate reductase activity. Applied Microbiology and Biotechnology, 100: 3235-3244.
47. Kamal Deep, Abhijit Poddar and Subrata K. Das^*. 2016. Cloning, overexpression and characterization of halostable, solvent tolerant novel β-endoglucanase from a marine bacterium Photobacterium panuliri LBS5^T (DSM 27646^T). Applied Biochemistry and Biotechnology, 178: 695-709
48. Jhasketan Badhai, Tarini Shankar Ghosh and Subrata K Das^*. 2015. Taxonomic and functional characteristics of microbial communities and their correlation with physicochemical properties of four geothermal springs in Odisha, India. Frontiers in Microbiology, 6: 936. doi: 10.3389/fmicb.2016.00936.
49. Rinchen T. Lepcha, Abhijit Poddar, Peter Schumann and Subrata K. Das*.  2015. Comparative 16S rRNA signatures and multilocus sequence analysis for the genus Salinicola and description of Salinicola acroporae sp. nov. isolated from coral Acropora digitifera. Antonie van Leeuwenhoek, 108: 59–73.
50. Prabla Kumari, Abhijit Poddar, Peter Schumann and Subrata K. Das^*. 2014. Vibrio panuliri sp. nov., a marine bacterium isolated from lobster, Panulirus penicillatus and transfer of V. ponticus from Scophthalmi clade to the newly proposed Ponticus clade. Research in Microbiology, 165: 826-835. 
51. Abhijit Poddar, Rinchen T Lepcha, Debasish Mukherjee, Dhananjay Bhattacharyya and Subrata K. Das*. 2014. Comparative analysis of 16S rRNA signature sequences of the genus Idiomarina and Idiomarina woesei nov., a novel marine bacterium isolated from Andaman Sea. Research in Microbiology. 165: 501-507.
52. Kamal Deep, Abhijit Poddar and Subrata K. Das*. 2014.  Photobacterium panuliri sp. nov., an alkalitolerant marine bacterium isolated from eggs of spiny lobster, Panulirus penicillatus from Andaman Sea. Current Microbiology. 69: 660-668.
53. Abhijit Poddar, Rinchen T Lepcha and Subrata K. Das*. 2014. Taxonomic study of the genus Tepidiphilus: Transfer of Petrobacter succinatimandens to the genus Tepidiphilus as Tepidiphilus succinatimandens comb. nov.; emended description of genus Tepidiphilus and description of Tepidiphilus thermophilus sp. nov. isolated from a terrestrial hot spring in India. International Journal of Systematic and Evolutionary Microbiology. 64: 228-235.
54. Prabla Kumari, Abhijit Poddar and Subrata K. Das*. 2014. Marinomonas fungiae sp. nov., isolated from a coral Fungia echinata from Andaman Sea. International Journal of Systematic and Evolutionary Microbiology. 64: 487-494.
55. Kamal Deep, Abhijit Poddar and Subrata K. Das. 2013. Anoxybacillus suryakundensis sp. nov, a moderately thermophilic, alkalitolerant bacterium isolated from hot spring at Jharkhand, India. PLoS One. 8 (12). e85493. doi: 10.1371/journal.pone.0085493.
56. Prabla Kumari, Saumya Bandyopadhyay and Subrata K Das*. 2013. Microbacterium oryzae nov., isolated from rice field. International Journal of Systematic and Evolutionary Microbiology. 63: 2442-2449.
57. Saumya Bandyopadhyay, Peter Schumann and Subrata K. Das*. 2013. Pannonibacter indica  nov., a highly arsenate-tolerant bacterium isolated from a hot spring in India. Archives of Microbiology. 195: 1-8.
58. Jhasketan Badhai, Prabla Kumari, P. Krishnan, T. Ramamurthy and Subrata K Das*. 2013. Presence of SXT integrating conjugative element in marine bacteria isolated from the mucus of the coral Fungia echinata from Andaman Sea. FEMS Microbiology Letters. 338: 118-123.
59. Madhuban Gopal, Debashis Dutta, Sunil Kumar Jha, Shobhita Kalra, Saumya Bandyopadhyay and Subrata K Das. 2011. Biodegradation of imidacloprid and metribuzin by Burkholderia cepacia strain CH9. Pesticide Research Journal. 23: 36-40.
60. Kunwar Digvijay Narayan, Shachindra K Pandey and Subrata K. Das. 2010. Characterization of Comamonas thiooxidans sp. nov and the comparison of thiosulfate oxidation with Comamonas testosteroni and Comamonas composti. Current Microbiology. 61: 248–253.
61. Madhuban Gopal, Ekta Mishra, Subrata K Das, S.C. Chatterjee, U.R. Sangle and O.M. Bambawale. 2010. Growth inhibition of different pathogenic fungi by actinomycetes and its identification by 16S rRNA. Indian Phytopathology. 63: 137-140.
62. Jyoti, Kunwar Digvijay Narayan and Subrata K. Das. 2010. Gulbenkiania indica sp. nov., isolated from a sulfur spring. Int. J. Syst. Evol. Microbiol. 60: 1052-1055.
63. Digvijay Panday and Subrata K. Das. 2010. Chelatococcus sambhunathii sp. nov., a moderately thermophilic alphaproteobacterium isolated from a hot spring sediment. Int. J. Syst. Evol. Microbiol. 60: 861-865.
64. Shachindra K. Pandey, Kunwar Digvijay Narayan, Saumya Bandyopadhyay, Kinshuk C. Nayak and Subrata K. Das. 2009. Thiosulfate oxidation by Comamonas sp. S23 isolated from a sulfur spring. Current Microbiology. 58: 516-521.
65. Sujogya K. Panda, V. Jyoti,Bhaskar Bhadra, Kinshuk C. Nayak, Sisinthy Shivaji, Fred A. Rainey and Subrata K. Das. 2009. Thiomonas bhubaneswarensis sp. nov., a novel obligately mixotrophic, moderately thermophilic, thiosulfate oxidizing bacterium. Int. J. Syst. Evol. Microbiol. 59: 2171- 2175.
66. Madhuban Gopal, Sunil Kumar Jha, Ram Niwas, Livleen Shukla and Subrata K. Das. 2008. Degradation of Cypermethrin by Pseudomonas stutzeri. Pesticide Research Journal. 20: 275-278.
67. Minakshi Patra, Shachindra K. Pandey, Durg V. Singh, T. Ramamurthy, Subrata K. Das. 2007. Characterization of cytotoxin producing Aeromonas caviae (strain HT10) isolated from a sulfur spring in Orissa, India. Letters in Applied Microbiology. 44: 338-341.
68. Nirmali Saikia, Subrata K. Das, Bharat K.C. Patel, Ram Niwas, Aqbal Singh, and Madhuban Gopal. 2005. Biodegradation of Beta cyfluthrin by Pseudomonas stutzeri strain S1. Biodegradation. 16: 581-589.
69. Subrata K. Das. 2005. Genus Bosea. In Kreig, Staley and Brenner (Editors), Bergey’s Manual of Systematic Bacteriology, 2^nd Edition. Vol 2. The Williams & Wilkins Co., Baltimore, pp. 459-461.
70. Hauwaerts, G. Alexandre, Subrata K. Das, J. Vanderleyden, I.B. Zhulin. 2002. A major chemotaxis gene cluster in Azospirillum brasilense and relatioships between chemotaxis operons in alpha-proteobacteria. FEMS Microbiology Lett. 208: 61-70.
71. Dutta, S. Ghosh, A.K. Maiti, Subrata K. Das, D.P. Modok, P .K. Chakraborty and P.K. Roy. 1998. Biodegradation of organochlorine pesticides by soil bacteria. Journal of Scientific and Industrial Research. 57: 838-845. IF:7.0
72. Subrata K. Das and Ajit K. Mishra. 1996. Transposon mutagenesis affecting thiosulfate oxidation in Bosea thiooxidans, a chemolithoheterotrophic bacterium. Journal of Bacteriology. 178: 3628-3633.
73. Subrata K. Das, Ajit K. Mishra, B.J. Tindall, F. A. Rainey and E. Stackebrandt. 1996. Oxidation of thiosulfate by a new bacterium, Bosea thiooxidans (BI-42) gen, nov., sp. nov,: Analysis of phylogeny based on chemotaxonomy and 16S ribosomal DNA sequencing. Int. J. Syst. Bacteriol. 46: 981-987.

Microbial Genomics, Gene Function and Regulation

My laboratory is working on microbial genomics towards translational research. In this regard, useful microbes with biotechnological potential have been characterized from hot springs and marine environments for bioprospecting of novel genes and metabolic pathways. In this endeavour, we have characterized an environmental Bordetella isolate as a potential resource for the evolution of Bordetella bronchiseptica hybrid strain for the development of whole cell livestock vaccine. Further, we are using metagenomic approach to unveil the community composition and functional correlation of microbiome in pristine environment. Following polyphasic approach, 20 new microbes have been discovered from unmanaged ecosystem. Our future goal to address the genome scale reconstruction of metabolic model with these microbes for the functional analysis of genes involved in synthesis of metabolites, pharmaceuticals and ecological adaptation.

No Current Positions Available.