1. Registered Veterinary Practitioner of the Odisha Veterinary Council
2. Registered Veterinary Practitioner of the Veterinary Council of India
3. Ramalingaswami Fellowship, Department of Biotechnology, India, 2012-2017
4. Harris Award in Cancer Research, UNMC, NE, USA, 2010
5. Harris Award in Cancer Research, UNMC, NE, USA, 2009
6. Pelotonia Postdoctoral Fellowship Ohio State University, Comprehensive Cancer Centre, Columbus, Ohio, USA , 2011- 2012
7. Predoctoral Prostate Cancer Research Training Award, Department of Defence, USA, 2009- 2010
8. University student fellowship, UNMC, NE, USA, 2008-2010
9. Research assistantship, UNMC, NE, USA, 2005-2008

Our lab focuses on understanding the tumor microenvironment (TME) of pancreatic and prostate cancers, with emphasis on immune regulation, stromal interactions, and infection-driven inflammation. We develop and use clinically relevant animal models and patient-derived samples to explore mechanisms of cancer progression and identify novel therapeutic strategies, including drug repurposing and immunomodulation. In parallel, we investigate the roles of probiotics and the microbiota in health and disease, advancing indigenous and marine probiotic strains as potential bio-therapeutics. Our expertise in animal models also enabled critical contributions to COVID-19 research and therapeutic development.

Understanding and targeting the pancreatic cancer tumor microenvironment

One of the major focuses of the lab is to elucidate the cellular and molecular intricacies of the Pancreatic Ductal Adenocarcinoma (PDAC) tumor microenvironment (TME) and to evaluate potential TME-targeting therapies using clinically relevant animal models and human patient-derived cells and/or tissue samples. Immune-competent animal models are potential tools for TME-related studies. In this regard our lab has characterised and used a hamster pancreatic cancer cell line (HapT1) -derived syngeneic PC tumor model (Oncotarget, 2016). The findings of this study motivated us to investigate the role of macrophage migration inhibitory factor (MIF) in PDAC. The findings of our study showed the pro-tumorigenic role of MIF expressed by both cancer cells and cancer associated fibroblasts (CAFs) (Scientific reports 2019; FASEB J., 2022). Recently, our lab has also established well-characterized multiple syngeneic mouse pancreatic cancer cell lines derived from genetically modified mouse models. These cell line-derived animal models are helping us address multiple questions about TME and its targeted therapy. Our lab has a significant interest in identifying immunomodulators, and in this regard, we are exploring novel approaches using existing drugs and/or novel drug candidates that could be useful as therapeutic agents. One of our studies showed that gemcitabine induces polarization of mouse peritoneal macrophages toward an M1-like phenotype, with antitumor effects via reactive oxygen species production (Clin Exp Metastasis, 2022). Further, we reported that statins counteract gemcitabine-induced PD-L1 expression in pancreatic cancer-associated fibroblasts and cancer cells, thereby improving cancer therapeutic outcomes (Cancer Immunol Immunother, 2023). Our efforts on drug repurposing revealed that Fluvastatin can be used as radio sensitizer to suppress tumor-associated fibrosis (Lab Invest, 2021). One of our lab’s interests was to investigate the roles of the cancer-testis antigens MAGEA3 and ATAD2 in PDAC. Our study found that MAGEA3 overexpression in pancreatic cancer cells confers a survival advantage (J Exp Clin Cancer Res, 2019). Through collaborative studies, we also found that MicroRNA-217 modulates pancreatic cancer progression via targeting ATAD2 (Life Sciences, 2022). These findings have provided a rationale for the design of novel therapeutics against PDAC. Currently, we are conducting two major studies to elucidate the heterogeneity of PDAC-associated CAFs and to understand the role of different CAF subpopulations in immunomodulation.

Investigating bacteria and their derived products on cancer pathogenesis and response to therapy

Our lab is interested in understanding and targeting inflammation and/or infection-mediated events in prostate and pancreatic cancers. Like many other cancers, prostate cancer patients are susceptible to various infections. Our work demonstrated that TLR4 activation by lipopolysaccharide confers a survival advantage to growth factor-deprived prostate cancer cells, emphasizing the critical role of inflammation and innate immune signalling in cancer cell survival (Prostate, 2015). Another study revealed that lipopolysaccharide (LPS) enhances prostate cancer metastasis potentially through NF-κB activation, further highlighting the link between infection-driven inflammation and cancer progression (Prostate, 2018). Further, our study found that Escherichia coli is commonly present in benign prostate hyperplasia-associated microbiota, which induces inflammation and DNA damage in prostate epithelial cells, linking infection to cancer development (Prostate, 2020). Currently, we are investigating the role of bacteria and their derived products in the cancer TME, specifically in immune cells, and their consequences on the response to various therapeutics.

Isolation, characterisation and use of probiotics for animal/human health and diseases

Apart from cancer biology, our lab has emerged as a key contributor in investigating the role of indigenous probiotics in human health, disease prevention, and therapy. We initially participated in the ILS flagship project on Tribal Health and Nutrition, funded by DBT, where our group isolated potential probiotic strains from tribal stool samples and traditional fermented beverages.  This work led to the comprehensive characterization of promising indigenous probiotic strains, including Ligilactobacillus salivarius F14, Lactiplantibacillus plantarum ILSF15, and Levilactobacillus brevis ILSH3, highlighting their potential as novel biotherapeutics (World J Microbiol Biotechnol., 2023; Gene, 2024; Probiotics Antimicrob Proteins, 2024). Investigation of these strains provided critical insights into the genomic basis of probiotic function, safety, and their adaptability to human gut environments while also demonstrating their antimicrobial, anti-cholesterol, and immunomodulatory properties. Our ongoing research is evaluating the preventive and/or therapeutic potential of these strains in models of non-alcoholic fatty liver disease and pancreatic cancer (Cell Mol Gastroenterol Hepatol, 2025). Our lab is also an active participant in the multi-institutional Himalayan Bio-mission Project, funded by DBT. In this project, our group is conducting a toxicological assessment of functional food formulations developed using characterized indigenous strains to ensure their safety and regulatory readiness for nutraceutical applications. Recently, our lab has been actively involved in a new institutional initiative for Marine Bioprospecting under the Deep Ocean Mission (supported by MoES), where we aim to isolate novel probiotic candidates from marine ecosystems for use in functional foods, biotherapeutics, aquaculture, and environmental bioremediation.

COVID-19 research and animal models

With our prior expertise in developing clinically relevant animal models, our group played a pivotal role during the COVID-19 pandemic. Supported by BIRAC funding, we established the BRIC-ILS ABSL-3 animal challenge platform for SARS-CoV-2 research, making our institute one of the few national facilities capable of developing and utilizing such models at a critical time. This platform not only enabled essential COVID-19 studies but also served multiple government and private organizations in a fee-for-service mode. Our research contributed to understanding SARS-CoV-2 biology, transmission, and pathogenesis (FASEB J., 2021; Eur J Pharmacol., 2023; Indian Patent: 202231071195). Building on this foundation, we are now engaged in identifying and validating potential drug candidates for COVID-19-associated lung fibrosis, leveraging both in vitro and in vivo models.

Publications from ILS, Bhubaneswar

Original research articles

1. Parida D, Mishra S, Mohapatra AP, Sethi M, Das KR, Kar S, Suresh V, Bhagat SJ, Mohapatra A, Halder S, Senapati S. Probiotics Mitigate High-cholesterol Diet-driven Fatty Liver and Pancreatic Cancer by Restoring Macrophage Homeostasis. Cell Mol Gastroenterol Hepatol. 2025 Oct 18;20(3):101644.
2. Arjun OK, Sethi M, Parida D, Dash J, Kumar Das S, Prakash T, Senapati S. Comprehensive physiological and genomic characterization of a potential probiotic strain, Lactiplantibacillus plantarum ILSF15, isolated from the gut of tribes of Odisha, India. Gene. 2024 Dec 30;931:148882. doi: 10.1016/j.gene.2024.148882.
3. Sethi M, Ok A, Dash J, Parida D, Kar S, Mishra S, Minz AP, Padhi A, Das KR, Pradhan B, Prakash T, Senapati S. Whole Genome Mining and Characterization of a New Probiotic Strain Levilactobacillus brevis ILSH3 from Handia: An Ethnic Fermented Beverage of Odisha, India. Probiotics Antimicrob Proteins. 2024 Feb 12. doi: 10.1007/s12602-024-10217-3.
4. Minz AP, Mohapatra D, Dutta M, Sethi M, Parida D, Mohapatra AP, Mishra S, Kar S, Sasmal PK, Senapati S. Statins abrogate gemcitabine-induced PD-L1 expression in pancreatic cancer-associated fibroblasts and cancer cells with improved therapeutic outcome. Cancer Immunol Immunother. 2023 Dec;72(12):4261-4278.
5. Dash J, Sethi M, Deb S, Parida D, Kar S, Mahapatra S, Minz AP, Pradhan B, Prasad P, Senapati S. Biochemical, functional and genomic characterization of a new probiotic Ligilactobacillus salivarius F14 from the gut of tribes of Odisha. World J Microbiol Biotechnol. 2023 Apr 27;39(7):171.
6. Suresh V, Behera P, Parida D, Mohapatra AP, Das SK, Kumari S, Avula K, Mohapatra A, Syed GH, Senapati S. Therapeutic role of N-acetyl cysteine (NAC) for the treatment and/or management of SARS-CoV2-induced lung damage in hamster model. Eur J Pharmacol. 2023 Jan 5;938:175392.
7.  Jha A, Ahad A, Mishra GP, Sen K, Smita S, Minz AP, Biswas VK, Tripathy A, Senapati S, Gupta B, Acha-Orbea H, Raghav SK. SMRT and NCoR1 fine-tune inflammatory versus tolerogenic balance in dendritic cells by differentially regulating STAT3 signaling. Front Immunol. 2022 Sep 27;13:910705.
8. Suresh V, Dash P, Suklabaidya S, Murmu KC, Sasmal PK, Jogdand GM, Parida D, Sethi M, Das B, Mohapatra D, Saha S, Prasad P, Satoskar A, Senapati S. MIF confers survival advantage to pancreatic CAFs by suppressing interferon pathway-induced p53-dependent apoptosis. FASEB J. 2022 Aug;36(8):e22449. doi: 10.1096/fj.202101953R.
9. Minz AP, Das B, Mohapatra D, Suresh V, Mishra S, Senapati S. Gemcitabine induces polarization of mouse peritoneal macrophages towards M1-like and confers antitumor property by inducing ROS production. Clin Exp Metastasis. 2022 Jul 15. doi: 10.1007/s10585-022-10178-3.
10. Singh B, Avula K, Chatterjee S, Datey A, Ghosh A, De S, Keshry SS, Ghosh S, Suryawanshi AR, Dash R, Senapati S, Beuria TK, Prasad P, Raghav S, Swain R, Parida A, Hussain Syed G, Chattopadhyay S. Isolation and Characterization of Five Severe Acute Respiratory Syndrome Coronavirus 2 Strains of Different Clades and Lineages Circulating in Eastern India. Front Microbiol. 2022 Jun 30;13:856913.
11. Pradeepa, Suresh V, Senapati S, Chakraborty S. AKT inhibition sensitizes EVI1 expressing colon cancer cells to irinotecan therapy by regulating the Akt/mTOR axis. Cell Oncol (Dordr). 2022 Jul 14. doi: 10.1007/s13402-022-00690-9.
12. Dutta M, Das B, Mohapatra D, Behera P, Senapati S, Roychowdhury A. MicroRNA-217 modulates pancreatic cancer progression via targeting ATAD2. Life Sci. 2022 Jul 15;301:120592. (Co-corresponding author)
13. Sethi M, Mohanty V, Mishra S, Dash J, Mahapatra S, Parida D, Mohapatra AP, Mishra R, Prasad P, Parida A, Senapati S. Targeted (PCR-based) screening of antibiotic resistance genes’ prevalence in the gut microbiota of tribal people of Nabarangpur, Odisha, India. Lett Appl Microbiol. 2021 Dec 27. doi: 10.1111/lam.13642.
14. Mohapatra D, Das B, Suresh V, Parida D, Minz AP, Nayak U, Mohapatra AP, Swain RK, Senapati S. Fluvastatin sensitizes pancreatic cancer cells toward radiation therapy and suppresses radiation- and/or TGF-β-induced tumor-associated fibrosis. Lab Invest. 2021 Nov 12. doi: 10.1038/s41374-021-00690-7
15. Pradeepa, Suresh V, Singh VK, Nayak KB, Senapati S, Chakraborty S. EVI1 promotes metastasis by downregulating TIMP2 in metastatic colon and breast cancer cells. Int J Biochem Cell Biol. 2021 Nov 18;142:106118.
16. Bishnoi S, Kumari A, Rehman S, Minz A, Senapati S, Nayak D, Gupta S. Fusogenic Viral Protein-Based Near-Infrared Active Nanocarriers for Biomedical Imaging. ACS Biomater Sci Eng. 2021 Jul 12;7(7):3351-3360.
17. Suresh V*, Mohanty V*, Avula K*, Ghosh A, Singh B, R.  Kumar R, Parida D, Suryawanshi AR, Raghav KS, Chattopadhyay S, Prasad P, Swain RK, Dash R, Parida A, Syed GH, Senapati S.  Quantitative proteomics of hamster lung tissues infected with SARS-CoV-2 reveal host-factors having implication in the disease pathogenesis and severity. FASEB J. 2021 Jul;35(7):e21713.
18. Suresh V, Parida D, Minz AP, Sethi M, Sahoo BS, Senapati S. Tissue Distribution of ACE2 Protein in Syrian Golden Hamster (Mesocricetus auratus) and Its Possible Implications in SARS-CoV-2 Related Studies. Front Pharmacol. 2021 Jan 14;11:579330.
19. Raghav S, Ghosh A, Turuk J, Kumar S, Jha A, Madhulika S, Priyadarshini M, Biswas VK, Shyamli PS, Singh B, Singh N, Singh D, Datey A, Avula K, Smita S, Sabat J, Bhattacharya D, Kshatri JS, Vasudevan D, Suryawanshi A, Dash R, Senapati S, Beuria TK, Swain R, Chattopadhyay S, Syed GH, Dixit A, Prasad P; Odisha COVID-19 Study Group; ILS COVID-19 Team, Pati S, Parida A. Analysis of Indian SARS-CoV-2 Genomes Reveals Prevalence of D614G Mutation in Spike Protein Predicting an Increase in Interaction With TMPRSS2 and Virus Infectivity. Front Microbiol. 2020 Nov 23;11:594928.
20. Senapati S*, Kshatri SJ*, Prasad P*, Turuk J, Pati S, Parida A. A pilot study to investigate the fecal dissemination of SARS-CoV-2 virus genome in COVID-19 patients in Odisha, India. medRxiv 2020.05.26.20113167 (preprint submission)
21. Selim AM, Das M, Senapati SK, Jena GR, Mishra C, Nath I, Senapati S, Sethi M. Molecular detection of Theileria annulata infection in cattle by conventional PCR and quantitative real time PCR in India. J Parasit Dis. 2021 Mar;45(1):72-77.
22. Paul P, Patel P, Verma SK, Mishra P, Sahu BR, Panda PK, Kushwaha GS, Senapati S, Misra N, Suar M. The Hha-TomB toxin-antitoxin module in Salmonella enterica serovar Typhimurium limits its intracellular survival profile and regulates host immune response. Cell Biol Toxicol. 2021 Mar 2.
23. Katoch A, Jamwal VL, Faheem MM, Kumar S, Senapati S, Yadav G, Gandhi SG, Goswami A. Overlapping targets exist between the Par-4 and miR-200c axis which regulate EMT and proliferation of pancreatic cancer cells. Transl Oncol. 2021 Jan;14(1):100879.
24. Jain S*, Samal AG* , Das B*, Pradhan B, Sahu N_, Mohapatra D , Behera PK, Satpathi PS, Mohanty AK, Satpathi S, Senapati S. Escherichia coli, a common constituent of benign prostate hyperplasia (BPH)-associated microbiota induces inflammation and DNA damage in prostate epithelial cells. (2020) Prostate. Aug 24. doi: 10.1002/pros.24063
25. Das, B, Senapati, S. Functional and mechanistic study reveals MAGEA3 as a pro-survival factor in pancreatic cancer cells. (2019) J Exp Clin Cancer Res. Jul 8;38(1):294
26. Sharma GP, Gurung SK, Inam A, Nigam L, Bist A, Mohapatra D, Senapati S, Subbarao N, Azam A, Mondal N. CID-6033590 inhibits p38MAPK pathway and induces S-phase cell cycle arrest and apoptosis in DU145 and PC-3 cells. Toxicol In Vitro. 2019 Oct;60:420-436.
27. Suresh V*, Sundaram R*, Dash P*, Sabat SC, Mohapatra D, Mohanty S, Vasudevan D, Senapati S. Macrophage migration inhibitory factor of Syrian golden hamster shares structural and functional similarity with human counterpart and promotes pancreatic cancer. (2019) Scientific Reports 9: 15507.
28. Izhar S, Gupta T, Minz AP, Senapati S, Panday AK. Influence of regional and long range transport air masses on fog water composition, contribution and toxicological response at Indo Gangetic Plain. Atmospheric Environment. Volume 214, 1 October 2019, 116888
29. Guha, D., Banerjee, A,. Mukherjee, R., Pradhan, B., Peneva, M., Aleksandrov, G., Suklabaidya, S., Senapati, S., Aich, P.: A Probiotic formulation containing Lactobacillus bulgaricus DWT1 inhibits tumor growth by activating pro-inflammatory responses in macrophages. Journal of Functional Foods. Volume 56, May 2019, Pages 232-245
30. Jain S, Dash P*, Minz A*, Satpathi S, Samal AG, Behera PK, Satpathi PS, Senapati S. Lipopolysaccharide (LPS) enhances prostate cancer metastasis potentially through NF-κB activation and recurrent dexamethasone administration fails to suppress it in vivo. (2018) Prostate. September 27
31. Jena KK, Kolapalli SP, Mehto S, Nath P, Das B, Sahoo PK, Ahad A, Syed GH, Raghav SK, Senapati S, Chauhan S, Chauhan S. TRIM16 controls assembly and degradation of protein aggregates by modulating the p62-NRF2 axis and autophagy. (2018) EMBO J. Aug 24.
32. Patra D, Mukherjee S, Chakraborty I, Dash TK, Senapati S, Bhattacharyya R, and Shunmugam R.Iron(III) Coordinated Polymeric Nanomaterial: A Next-Generation Theranostic Agent for High-Resolution T1-Weighted Magnetic Resonance Imaging and Anticancer Drug Delivery. (2018) ACS Biomater. Sci. Eng. 4 (5), pp 1738–1749
33. Pradhan B, Guha D, Naik AK, Banerjee A, Tambat S, Chawla S, Senapati S, Aich P. Probiotics L. acidophilus and B. clausii Modulate Gut Microbiota in Th1- and Th2-Biased Mice to Ameliorate Salmonella Typhimurium-Induced Diarrhea. (2018) Probiotics Antimicrob Proteins. Jun 16.
34. Katoch A, Suklabaidya S, Chakraborty S, Nayak D, Rasool RU, Sharma D, Mukherjee D, Faheem MM, Kumar A, Sharma PR, Senapati S, Kumar LD, Goswami A. Dual Role of Par-4 in abrogation of EMT and switching on Mesenchymal to Epithelial Transition (MET) in metastatic pancreatic cancer cells. (2018) Mol Carcinog. Apr 19
35. Kumari K, Das B, Adhya A, Chaudhary S, Senapati S, Mishra SK. Nicotine associated breast cancer in smokers is mediated through high level of EZH2 expression which can be reversed by methyltransferase inhibitor DZNepA . (2018) Cell Death Dis. Feb 2;9(2):152
36. Mahapatra P, Jain S, Shrestha S, Senapati S, Puppala S. Ambient endotoxin in PM10 and association with inflammatory activity, air pollutants, and meteorology, in Chitwan, Nepal. (2018) Sci Total Environ. Mar 15;618:1331-1342
37. Suklabaidya S, Das B, Ali SA, Jain S, Swaminathan S, Mohanty AK, Panda SK, Dash P, Chakraborty S, Batra SK, Senapati S. Characterization and use of HapT1-derived homologous tumors as a preclinical model to evaluate therapeutic efficacy of drugs against pancreatic tumor desmoplasia. (2016) Oncotarget. Jul 5;7(27):41825-41842
38. Barman PK, Mukherjee R, Prusty BK, Suklabaidya S, Senapati S, Ravindran B.Chitohexaose protects against acetaminophen-induced hepatotoxicity in mice. (2016) Cell Death & Disease. May 12;7
39. Jain S, Suklabaidya S, Das B, Raghav SK, Batra SK, Senapati S. TLR4 activation by lipopolysaccharide confers survival advantage to growth factor deprived prostate cancer cells. (2015) Prostate. Jul 1;75(10):1020-33.
40. Sarkar S, Jain S, Rai V, Sahoo DK, Raha S, Suklabaidya S, Senapati S, Rangnekar VM, Maiti IB, Dey N. Plant-derived SAC domain of PAR-4 (Prostate Apoptosis Response 4) exhibits growth inhibitory effects in prostate cancer cells. (2015) Front Plant Sci. Oct 7;6:822.
41. Maiti S, Patro S, Purohit S, Jain S, Senapati S, Dey N. Effective control of Salmonella infections by employing combinations of recombinant antimicrobial human β-defensins hBD-1 and hBD-2. (2014) Antimicrob Agents Chemother. Nov;58(11):6896-903.
42. Sahu PK*, Das B*, Sahoo L, Senapati S, Nayak GD. Genetic relationship and population structure of three Indian local chicken populations as revealed by mtDNA D-loop. (2015) Mitochondrial DNA. Jul 10:1-3.
43. Singh A, Jain S, Senapati S, Verma RS and Sahoo SK. Magnetic nanoparticles labeled mesenchymal stem cells: A pragmatic solution towards targeted cancer theranostics. (2015) Advanced Healthcare Materials. Sep 1
44. Pati, S. Jain, M. Behera, A.P. Acharya, S.K. Panda, S. Senapati. X-Gal staining of canine skin tissues: a technique with multiple possible applications. (2014) Journal of Natural Science, Biology and Medicine. July;5:245-249

Patents

Senapati S, Mohapatra AP, Parida D : Design and use of disposable animal isolator for in vivo imaging of small animals in micro CT systems placed inside bio-containment facility (Indian Patent Number: 583796; Granted)

Reviews and Book Chapters

1. Kar S, Panda S, Mishra S, Mohapatra AP and Senapati S. “3D bioprinting for recapitulation of tumour microenvironment”. 3D Bioprinting in Cancer Applications, Academic Press, 30 Jun 20 (Book Chapter)
2. Mishra S, Dash J , Mohapatra A, Sethi M, Senapati S. Probiotics based anticancer immunity in pancreatic cancer. Anticancer Immunity: Reviewing the Potential of Probiotics , pp 52-72, June 2023 (Book Chapter)
3. Mohapatra AP, Parida D, Mohapatra D, Nayak U, Swain RK, Senapati S. Zebrafish Larvae as a Model to Evaluate Potential Radiosensitizers or Protectors. J Vis Exp. 2022 Aug 25;(186). doi: 10.3791/64233.
4. Mohapatra D, Mohapatra A.P, Sahoo A.K, and Senapati, S. In-vitro and in-vivo models for the identification and validation of radioprotectors and radiosensitizers. Journal of Applied Biology & Biotechnology Vol. 11(2), pp. 94-101, Mar-Apr, 2023
5. Tripathy A, Dash J, Kancharla S, Kolli P, Mahajan D, Senapati S, Jena MK. Probiotics: A Promising Candidate for Management of Colorectal Cancer. Cancers (Basel). 2021 Jun 25;13(13):3178.
6. Senapati S, Dash J, Sethi M, Chakraborty S (2020) Bioengineered probiotics to control SARS-CoV-2 infection. Research Ideas and Outcomes 6: e54802. https://doi.org/10.3897/rio.6.e54802
7. Suklabaidya S*, Dash P*, Das B, Suresh V, Sasmal PK, Senapati S. Experimental models of pancreatic cancer desmoplasia. Lab Invest.2018 Jan;98(1):27-40 
8. Senapati S, Chakraborty S, Nath I, Panda SK, Patra RC and Batra SK. Non- Human Prostate Cancer Cell Lines. Oncology, Gastroenterology and Hepatology reports. 2012; 1(1):15-24
9. Das B, Senapati S. Immunological and functional aspects of MAGEA3 cancer/testis antigen. Adv Protein Chem Struct Biol. 2021;125:121-147. (Book Chapter)
10. Senapati S, Singh RK and Batra SK. Tumor microenvironment: Blood vascular system in cancer metastasis. In: Systems Biology of Cancer, Editor: Sam Thiagalingam, Cambridge University Press London. (Book Chapter)

Extramural funding