Bachelor of Science: Banaras Hindu University (1982)
Master of Science: Banaras Hindu University (1985)
PhD: Banaras Hindu University (1991)
Dr. D.V. Singh in association with international collaborators has successfully shown the clonal relationships between clinical and environmental isolates of V. cholerae indicating aquatic environment serve as a reservoir and transmission of the disease cholera. His group is the first to report occurrence of SXT ICE, dfr18, sulII, strAB and aadA5 genes in environmental V. cholerae O1, and non-O1, and non-O139 strains isolated before 1992 from Varanasi, India, and that showed sequence homology with other bacterial species, suggesting acquisition of SXT ICEs and antibiotic resistance genes occurred from other bacteria. His observation showing loss of the complete and/or partial loss of phage array arranged in tandem lead to understanding of emergence and evolution of variant of V. cholerae strains. Dr. Singh has developed number of multiplex PCRs for rapid detection of virulence genes, serogroups, SXT and antibiotic resistance genes. While working on staphylococcus, he developed multiplex PCR for rapid detection of antibiotic, cadmium/zinc and antiseptic resistance in PVL-positive and -negative S. aureus and CoNS. He found that multi-drug resistant S haemolyticus strains produced biofilm and matrix belongs mostly to eDNA and/or protein. Dr. Singh has 74 publications in international and national ISI Journals – many in collaboration with external scientists.
Awards:
ASM Indo-US Visiting Research Professorship (2008)
GBF International Training Fellowship (2002)
Visiting Fellowship of UMSM, Baltimore, USA (1998)
UNESCO Short – Term Training Fellowship in Biotechnology (1998)
AMI Best poster award (1998)
Recognition:
Elected Fellow: Biotech Research Society (India) (2004)
Member: Peer Review committee Department of Biotechnology, IIT, Guwahati (2014)
Member: Institutional Animal Ethics Committee, OBPI, Bhubaneswar (2005-contd.)
Member: Doctoral Committee, NISER, Bhubaneswar (2015-contd.)
Understanding the role of genetic element in eDNA release and biofilm development in Staphylococci
Our research program focuses on to characterize Staphylococcus spp, to identify the key functional and regulatory genes involved in biofilm development and to examine role of biofilm in evolution, horizontal/ vertical gene transfer of antibiotic resistance and virulence genes and its effect on immunological responses in biofilm infections. The research initiative has the potential to understand biofilm development and to find suitable drug target and/or inhibitors to limit biofilm formation and antibiotic resistance/tolerance.
ICP1- phage and its role in rugosity and biofilm formation in V. cholerae O1
We plan to study how ICP1 phage genome exists in V. cholerae as an extra-chromosomal element and had a role in the enhancement of biofilm formation and virulence. To achieve this, we will use site-directed mutagenesis/ CRISPER-cas technique and conduct functional analysis of ompR, mscS, cic and Na+/proline symporter to establish their involvement in biofilm and rugose colony formation and in virulence. Studies on genetic analysis of CTX+ and CTX– strains of V. cholerae will continue to determine how horizontal transfer play a role in the evolution of new clone(s) of V. cholerae.
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Amol Kanampalliwar, SRF (Institutional) Email: amol [dot] kanampalliwar [at] gmail [dot] com; amol [dot] kanampalliwar [at] ils [dot] res [dot] in |
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Shifu Aggarwal, SRF (Institutional) Email: shifu [dot] agg [at] gmail [dot] com |
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Subhasree Saha, JRF (DST-Inspire)
Email: subhashree [dot] saha [dot] 2010 [at] gmail [dot] com |
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Swagata Bose, JRF (Institutional)
Email: swagatatitly122 [at] gmail [dot] com |
Jawed Ahmed, Senior Research Associate (CSIR)
Email: jawedalam81[at[gmail[dot].com |
Alumni: Doctoral students
Alumni: Post Doctoral Fellows
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DBT: Department of Biotechnology, Government of India
DST: Department of Science and Technology, Government of India
ICMR: Indian Council of Medical Research, Government of India
Name of Project | Funding Agency | Duration | Amount (in Rs.) |
A diagnostic panel for rapid detection of early-onset sepsis in high-risk neonates | DBT-ICMR | 2018-2020 | 18,94,000.00 |
Studies on antibiotic resistance, virulence pattern and biofilm formation among Staphylococcus haemolyticus strains isolated from ocular infection | DST | 2013-2016 | 33,55,600.00 |
Ocular infections caused by Staphylococcus species: Study of clinico-microbiologic features, virulence markers and genome profile | DST | 2009-2012 | 17,54,400.00 |
Molecular analysis of environmental Vibrio cholerae strains: relationships with clinical isolates | DST | 2007-2010 | 18,45,000.00 |
Purification and characterization of a new toxin produced by candidate cholera vaccine strains | ICMR | 2006-2008 | 7,30,056.00 |
Characterization of the new secretogenic toxin produced by Vibrio cholerae non-O1, non-O139 using panel of monoclonal antibodies | DST | 2000-2003 | 17,89,524.00 |
singhdv [at] ils [dot] res [dot] in, durg [dot] singh [at] gmail [dot] com
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Nalco Square, Bhubaneswar-751023, India
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+91 674 2300728
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+91 674 2304326(O) / +91 674 2300137/ 2301476 ext. 326
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