Development of novel promoter with enhanced activity
Position and Employment
Awards and Recognition
Development of Novel plant promoters
Our ongoing research activities involve the molecular characterization of pararetroviral promoters for designing efficient native promoters and/or chimeric promoters for enhanced gene expression in transgenic plants. The promoters so designed will be evaluated for the presence of salient cis-regulatory elements and the cognate trans-acting factors. The various DNA regulatory elements are assessed for their binding with transcription factors of the TGA and WRKY family. Our group is working on the analysis of these promoters in driving the expression of various biotic and abiotic stress response genes and genes of pharmaceutical importance in planta. We are also working to find elite miRNA responsible for plant growth and development. In addition, our group is also working to decipher the molecular and physiological role of WRKY TFs in pearl millet (Pennisetum Glaucum) under environmental stress conditions.
Deb, D., Shrestha, A., Sethi, L., Das, N. C., Rai, V., Das, A. B., Maiti, I. B., Dey, N. (2020). Transgenic tobacco expressing Medicago sativa Defensin (Msdef1) confers resistance to various phyto-pathogens. Nucleus. https://doi.org/10.1007/s13237-020-00307-2
Khan, A., Shrestha, A., Shaju, M., Panigrahi, K. C., & Dey, N. (2019). Identification of miRNA Targets by AtFT Overexpression in Tobacco. Plant Molecular Biology Reporter, 1-14.
Deb D., Dey N. (2019) Synthetic Salicylic Acid Inducible Recombinant Promoter for Translational Research. Journal of Biotechnology. 297, 9-18, ISSN 0168-1656.
Shrestha A., Khan A., Dey N. (2019) CRISPR-Cas9-Mediated Editing of the CYP82E4-Nicotine N-Demethylase (nnd) Gene in Tobacco Protoplasts. Journal of Plant Biochemistry & Physiology. 7:230.
Shrestha A., Khan A., Dey N. (2019) Cis–trans Engineering: Advances and Perspectives on Customized Transcriptional Regulation in Plants. Molecular Plant. 11:7, 886-898, ISSN 1674-2052.
Shrestha A., Khan A., Dey N. (2019) Identification of Novel Pararetroviral Promoters for Designing Efficient Plant Gene Expression Systems. Methods in Molecular Biology. Plant Innate Immunity, 978-1-4939-9457-1, 454350_1_En, Vo. 1991 (19)
Khan A., Shrestha A., Dey N. (2019) Biochemical and Molecular Characterization of Novel Pararetroviral Promoters in Plants. Methods in Molecular Biology. Plant Innate Immunity, 978-1-4939-9457-1, 454350_1_En, Vol. 1991 (20)
Deb D., Shrestha A., Maiti I B & Dey N (2018). Recombinant promoter (MUASCsV8CP) driven Totiviral Killer Protein 4 (KP4) imparts resistance against fungal pathogens in transgenic tobacco. Frontiers in Plant Science. 2018; doi: 10.3389/fpls.2018.00278
Khan A, Shrestha A, Bhuyan K, Maiti I B, & Dey N (2018). Structural characterization of a novel full-length transcript promoter from Horseradish Latent Virus (HRLV) and its transcriptional regulation by multiple stress responsive transcription factors. Plant molecular biology. 2018; 96 (1-2); DOI: 10.1007/s11103-017-0693-6. (Impact factor-3.356)
Sarkar S, Das A, Khandagale P, Maiti IB, Chattopadhyay S, Dey N. (2018). Interaction of Arabidopsis TGA3 and WRKY53 transcription factors on Cestrum yellow leaf curling virus (CmYLCV) promoter mediates salicylic acid-dependent gene expression in planta. Planta. 2018 Jan 1;247(1):181-99.DOI: 10.1007/s00425-017-2769-6 (Impact factor- 3.361)
Chatterjee A, Das NC, Raha S, Maiti IB, Shrestha A, Khan A, Acharya S, Dey N. (2017). Enrichment of apoplastic fluid with therapeutic recombinant protein for efficient biofarming. Biotechnology Progress. 2017 May;33(3):726-736. DOI: 10.1002/btpr.2461. (Impact factor 1.986)
Patel A, Dey N, Chaudhuri S, Pal A. (2017) Molecular and biochemical characterization of a Vigna mungo MAP kinase associated with Mungbean Yellow Mosaic India Virus infection and deciphering its role in restricting the virus multiplication. Plant Science. 2017 Sep1;262:127-40. (Impact factor- 4.148)
Sunita Patro, Deepak Kumar, Rajiv Ranjan, Indu B. Maiti, NrisinghaDey (2012) The Development of Efficient Plant Promoters for Transgene Expression Employing Plant Virus Promoters; Molecular Plant (2012) Doi: 10.1093/mp/sss028) (IF 6.126)
Soumitra Maiti, Sunita Patro, Sukumar Purohit, Sumeet Jain, Shantibhusan Senapati, Nrisingha Dey (2014) Effective control of Salmonella infections by employing combinations of recombinant antimicrobial human β-defensins hBD-1 and hBD-2 Antimicrobial Agents and Chemotherapy (2014) Doi: 10.1128/AAC.03628-14 (IF 4.55)
Rajiv Ranjan, Sunita Patro, Bhubaneswar Pradhan, Alok Kumar, Indu B Maiti, Nrisingha Dey (2012) Development and Functional Analysis of Novel Genetic Promoters Using DNA Shuffling, Hybridization and a Combination Thereof PLoS ONE (2012) Doi:1371/ journal.pone.0031931 (IF 4.41)
Deepak Kumar, Sunita Patro, Rajiv Ranjan, Dipak K Sahoo, Indu B. Maiti, Nrisingha Dey (2011) Development of useful recombinant promoter and its expression analysis in different plant cell using Confocal Laser Scanning Microscopy PLoS ONE (2011) Doi:10.1371/journal. pone. 0024627 (IF 4.411)
Jayadri Sekhar Ghosh, Shubho Chaudhuri , Nrisingha Dey, Amita Pal (2013) Functional characterization of a serine-threonine protein kinase from Bambusabalcooa that implicates in cellulose overproduction and superior quality fiber formation BMC Plant Biology (2013) Doi:10.1186/10.1186/1471-2229-13-128 (IF 4.36)
Shayan Sarkar, Sumeet Jain, Vineeta Rai, Dipak K. Sahoo, Sumita Raha, Sujit Suklabaidya, Shantibhusan Senapati, Vivek M. Rangnekar, Indu B. Maiti, Nrisingha Dey (2015) Plant-derived SAC domain of PAR-4 (Prostate Apoptosis Response 4) exhibits growth inhibitory effects in prostate cancer cells Frontiers iPlant Science (2015) Doi:10.3389/fpls.2015.00822 (IF 3.95)
Rajiv Ranjan, Nrisingha Dey (2012) Development of Vascular Tissue and Stress Inducible Hybrid Synthetic Promoters Through Dof-1 Motifs Rearrangement Cell Biochemistry and Biophysics (2012) Doi: 10.1007/s12013-012-9359-9 (IPF3.743)
Dipak Kumar Sahoo Nrisingha Dey, Indu B. Maiti (2014) pSiM24 is a novel versatile gene expression vector for transient assays as well as stable expression of foreign genes in plants PLoS One (2014) Doi:10.1371/journal.pone.0098988 (IF 3.534)
Joydeep Banerjee, Dipak Kumar Sahoo, Nrisingha Dey, Robert L. Houtz, Indu B. Maiti @014) An intergenic region shared by At4g35985 and At4g35987 in Arabidopsis thaliana is a tissue specific and stress inducible bidirectional promoter analyzed in transgenic arabidopsis and tobacco plants. PLoS One (2014) Doi:10.1371/journal.pone.0079622 (IF 3.543)
Dipak Kumar Sahoo, Shayan Sarkar, Sumita Raha, Indu B. Maiti, Nrisingha Dey (2014) Comparative analysis of synthetic DNA promoters for high-level gene expression in plants Planta (2014) Doi:10.1007/s00425-014-2135-x (IF 3.376)
Sefali Acharya,, Rajiv Ranjan,, Sitakanta Pattanaik,, Indu B. Maiti, Nrisingha Dey (2014) Efficient chimeric plant promoters derived from plant infecting viral promoter sequences Planta (2014) Doi: 10.1007/s00425-013-1973-2 (IF 3.376)
Kirti Mishra, Aditya P Dash, Bijay K Swain, Nrisingha Dey (2009) “Anti-malarial activities of Andrographis paniculata and Hedyotis corymbosa extracts and their combination with curcumin” Malaria Journal, 8:26 doi:10.1186/1475-2875-8-26 IF: 3.49
Nrisingha Dey, Shayan Sarkar, Sefali Acharya, Indu B. Maiti (2015) Synthetic promoters in planta Planta (2014) Doi: 10.1007/s00425-015-2377-2 (IF 3.36)
Sefali Acharya, Soumika Sengupta, Sunita Patro, Sukumar Purohit, Sabindra Samal, Indu B. Maiti, Nrisingha Dey (2013) Development of an intra-molecularly shuffled efficient chimeric plant promoterfrom plant infecting Mirabilis mosaic virus promoter sequence Biotechnology (2013) Doi:10.1016/j.jbiotec.2013.08.022 (IF 3.18)
Sunita Patro, Indu B. Maiti, NrisinghaDey (2013) Development of an efficient bi-directional promoter with tripartite enhancer employing three viral promoters Biotechnology (2013) Doi:10.1016/j.jbiotec.2012.11.009 (IF 3.18)
Vineeta Rai, Shayan Sarkar, Suresh Satpati, Nrisingha Dey (2015) Overexpression of human peroxisomal Enoyl-CoA delta isomerase2 HsPECI2, an ortholog of bamboo expressed during gregarious flowering alters salinity stress responses and polar lipid content in tobacco Functional Plant Biology(In Press) (IF 3.145)
Rajiv Ranjan,Sunita Patro, Sangeeta Kumari, Deepak Kumar, Indu B. Maiti, Nrisingha Dey (2011) Efficient chimeric promoters derived from full-length and sub-genomic transcript promoters of Figwort mosaic virus (FMV) Biotechnology (2011) Doi:10.1016/j.jbiotec.2011.01.015 (IF 3.045)
Bhubaneswar Pradhan,Afsar Raza Naqvi,Shradha Saraf,Sunil Kumar Mukherjee, Nrisingha Dey (2014) Prediction and characterization of Tomato leaf curl New Delhi virus (ToLCNDV) responsive novel microRNAs in Solanum lycopersicum Virus Research (2014) Doi:10.1016/j.virusres.2014.09.001 (IF 2.83)
Soumitra Maiti, Sunita Patro, Amita Pal, Nrisingha Dey (2014) Identification of a novel salicylic acid inducible endogenous plant promoter regulating expression of CYR1, a CC-NB-LRR type candidate disease resistance gene in Vigna mungo Plant Cell, Tissue and Organ Culture (2014) Doi: 10.1007/s11240-014-0616-z (IF 2.712)
Vineeta Rai, Nrisingha Dey (2012) Identification of programmed cell death related genes in bamboo Gene (2012) Doi:10.1016/j.gene.2011.01.004 (IF 2.443)
Deepak Kumar, Sunita Patro, Jayshis Ghosh, Abhimanyu Das,Indu B. Maiti, Nrisingha Dey (2012) Development of salicylic acid inducible minimal sub-genomic transcript promoter from Figwort mosaic virus with enhanced root- and leaf- activity using TGACG motif rearrangement Gene (2012) Doi:10.1016/j.gene.2012.04.053 (IF2.443)
Vineeta Rai,Jayadri Sekhar Ghosh, Amita Pal, Nrisingha Dey (2011) Identification of genes involved in bamboo fiber development Gene (2011) Doi:10.1016/j.gene.2012.01.018 (IF 2.443)
Joydeep Banerjee, Dipak Kumar Sahoo, Sumita Raha, Shayan Sarkar, Nrisingha Dey, Indu B. Maiti (2014) A Region Containing an as-1 Element of Dahlia Mosaic Virus (DaMV) Subgenomic Transcript Promoter Plays a Key Role in Green Tissue- and Root-Specific Expression in Plants Plant Molecular Biology Reporter (2014)Doi: 10.1007/s11105-014-0766-5 (IF 2.37)
Dipak k. Sahoo, Rajiv Ranjan, Deepak Kumar, Alok Kumar, Bhabani S.Sahoo, Sumita Raha, Indu B Maiti, Nrisingha Dey (2009) Analternative method of promoter assessment by confocal laser scanning microscopy Virol. Methods, doi:10.1016/j.jviromet.2009.06.01 IF: 2.27
Dipak Kumar Sahoo,Shayan Sarkar,Sumita Raha, Narayan Chandra Das, Joydeep Banerjee, Nrisingha Dey, Indu B. Maiti (2014) Analysis of Dahlia Mosaic Virus Full-length Transcript Promoter-Driven Gene Expression in Transgenic Plants Plant Molecular Biology Reporter (2014) (IF 2.37)
Kirti Mishra, DipjyotiChakraborty, Amita Pal, NrisinghaDey (2010) Plasmodium falciparum: In-vito interaction of quassin and neo-quassin with arsenate, a hemisuccinate derivative of artemisinin Experimental parasitological 124:421-427 (IF 1.957)
Sunita Patro, Soumitra Maiti, Santosh Kumar Panda, Nrisingha Dey (2014) Utilization of plant-derived recombinant human β-defensins (hBD-1 and hBD-2) for averting salmonellosis Transgenic Research (2014) Doi:10.1007/s11248-014-9847-3 (IF 2.32)
Vineeta Rai, Sefali Acharya, Nrisingha Dey (2012) Implications of Nanobiosensors in Agriculture Journal of Biomaterials and Nanobiotechnology, (2012) doi:10.4236/jbnb.2012.322039 (IF 1.79)
Samik Bhattacharyya, Jayadri S. Ghosh, Dipak K. Sahoo, NrisinghaDey, Amita Pal (2010) Screening of superior fiber-quality-traits among wild accessions of Bambusabalcooa : Efficient and non-invasive evaluation of fiber developmental stages Annals of Forest Sciences: 2010, 67 (6): 611 (IF 1.44)
Rai V, Ghosh JS, Dey N (2010) Isolation of total RNA from hard bamboo tissue rich in polyphenols and polysachharides for gene expression studies EJBiotech 2010, 13(5): 17 (IF 0.98)
Mondal, P., Dey, N., Dash, A. K., Chatterjee, A., Sahu, B.B., Panda, B., Maiti, I. B., and Sabat, S. C. (2007), “Structural and functional analysis of rice catalase-B gene promoter: presence of Dof and CAAT binding Site” Pant Biology Reporter:25 (1-2), 71-82; DOI: 10, 1007/S11105-007-00116. IF 0.91
As Postdoc and graduate student
Cao, X., Costa, L.M., Petit, C.B., Kbhaya, B., Dey, N., Perez, P., McCarty, D. R., Marcos, J. F. and Becraft, P. W. (2007) “Abscisic acid and stress signals induce viviparous 1 (vp1) exression in seed and vegetative tissues of maize”, Plant Physiol . 143(2):720-31.
Lai, J., Dey, N., C.-S., Bharti, A. K., Rudd, S., Mayer, K.F.X., Larkins, B., Becraft, P., and Messing, J. (2004) “Characterization of maize endosperm transcriptom and its comparison to the rice genome”, Genome Research 14, 1932-1937.
Becraft, W. P., Li, K., Dey, N., Yvonne, A., (2002) “The maize dek1 gene functions in embryonic patern formation and cell fate specification” Development 129, 5217-5225
Dey, N. and Maiti, I. B. (1999), “Structure and promoter/leader deletion analysis of mirabilis mosaic virus full-length transcript promoter in transgenic plant” Plant Molecular Biology, 40: 771-782.
Bhattacharyya, S., Dey, N. and Maiti, I. B. (2002), “Analysis of cis-sequence of subgenomic transcript promoter from the Figwort mosaic virus and comparison of promoter activity with the cauliflower mosaic virus promoters in monocot and dicot cells” Virus Research 90, 47
Pattanayak, S., Dey, N., Bhattacharyya, S. and Maiti, I.B. (2004), “Isolation of full-length transcript promoter from Strawberry vein banding virus (SVBV) and expression analysis by protoplasts transient assays and in transgenic plants”, Plant Science 167, 427-438.
Maiti, I.B., Dey, N., Dahlman, Douglas. L. and Webb, B. (2003) “Antibiosis-type resistance in transgenic plants expressing teratocyte secretary protein (TSP) gene from Hymenopteran Endoparasites (Microplitis Croceipes)” Plant Biotechnology, 1:209-219
Dey, N. and Maiti, I. B. (2002), “Promoter deletion and comparative analysis of the Mirabilis mosaic caulimovirus (MMV) sub-genomic transcript (Sgt) promoter in transgenic plants Transgenics 4, 35-53
Dey, N. and Maiti, I. B. (1999), “Further Characterization and expression analysis of Mirabilis Mosaic Caulimovirus (MMV) Full-length transcript promoter with single and double enhancer domains in transgenic plants” Transgenics, 3: 61-70.
Maiti, I.B., Lanken, C.V., Hong, Y., Dey, N. and Hunt. A.G. (1999) “Expression of multiple Virus-derived resistance determinants in transgenic plants does not lead to additive resistance properties” J. Plant Biochemistry and Biotechnology 8: 67-73.,
Dey, N. and Mandal R. K. (1993) “Characterisation of 2S albumin with nutritionally balanced aminoacid composition from the seeds of Chenopodium album and its antigenic homology with seed proteins of some Chenopodiaceae and Amaranthaceae species” Biochemistry and Molecular Biology International, 30: 149-157.
Ghosh SK, Dey N, Bhattacharya S and Mandal RK, Regeneration and transformation of Nasturtium indicum: a wild crucifer Current Science, 63: 44-46, 1992.
Ghosh SK, Dey N, Mandal RK, Dasgupta J and Maiti I B, Some physiological parameters and seed lipid composition
Patents filed from lab:
Patent 1 NOVEL SUB-GENOMIC TRANSCRIPT PROMOTER DNA FRAGMENTS AND A METHOD FOR OBTANING SUB-GENOMIC PROMOTER DNA FRAGMENTS FROM FIGWORT MOSIAC VIRUS (742/KOL/09 (INDIA) 15.05.2009; Published 26.08.2011(U/S 11A)
Patent 2 NOVEL PARARETROVIRUS BASED HYBRID PROMOTER DNA FRAGMENT (741/KOL/09 (INDIA) dt. 15.05.2009; Published 26.08.2011(U/S 11A)
Patent- 3 VASCULAR TISSUE SPECIFIC AND STRESS INDUCIBLE HYBRID PROMOTER IDEAL FOR PLANT DERIVED PHARMACEUTICAL PRODUCTION (1449/KOL/2011 (INDIA) 11.11.2011
Patent- 4 AN ARTIFICIAL DNA CONSTRUCT HAVING AN UNIQUE BIDIRECTIONAL GENETIC PROMOTER; 1407/KOL/2011 (INDIA) 03.11.2011; PCT/IN2012/000009 dt.04.01.2012
Patent- 5 A METHOD FOR ENHANCING ANTIMICROBIAL ACTIVITY OF PLANT AND BACTERIA DERIVED HUMAN BETA DEFENSIN-1 (HBD-1) AND HUMAN BETA DEFENSIN-2 (HBD-2); 1201/KOL/2012 (INDIA) dt.18.10.2012
Patent-6 A SALICYLIC ACID INDUCIBLE PROMOTER DNA FRAGMENT OBTAINED FROM FIGWORT MOSAIC VIRUS SUB-GENOMIC TRANSCRIPT PROMOTER AND A METHOD FOR PRODUCING THE SAME; 1408/KOL/2011 (INDIA) dt. 03.11.2011; PCT/IN2012/000008 dt. 04.01.2012
Patent 7 UNIQUE NUCLEIC ACID PROMOTERS FROM TWO OR MORE PROMOTER SEQUENCES; US Patent filling No. 287744v1_UK1902
Patent 8 A NOVEL FULL-LENGTH TRANSCRIPT PROMOTER DNA FRAGMENT FROM DALHIA MOSAIC VIRUS; 580/Kol/2014(INDIA)
Patent 9 A NOVEL SUB-GENOMIC TRANSCRIPT PROMOTER DNA FRAGMENT FROM DALHIA MOSAIC VIRUS; 578/Kol/2014 (India)
|DEEPAK KUMAR, PhD (2007-12) Ph. D. Awarded in 2013
Thesis Title: Pararetrovirus based hybrid promoters for enhanced expression of transgene
|RAJIV RANJAN. PhD (2008-13) Ph. D. Awarded in 2014
Thesis Title: Studies on some plant pararetrovirus promoters for developing hybrid synthetic promoters with enhanced activities
|VINEETA RAI, PhD (2009-15) Ph. D. Awarded in 2015
Thesis Title: Studies on characterization of genes (ESTs) associated with bamboo development
|SUNITA PATRO, PhD (2009-14) Ph. D. Awarded in 2015
Thesis Title: Strength and tissue specificity of chimeric promoters derived from full-length and sub-genomic transcript promoters from different pararetroviruses and their uses in translational research
|BHUBANESWAR PRADHAN, PhD (2010-2015) Ph. D. Awarded in 2015
Thesis Title: Isolation and Characterization of microRNA in plants under different biotic stress conditions
|SEFALI ACHARYA, PhD (2011-15) PhD awarded in 2017
Thesis Title: Designing and testing efficient promoters for enhanced molecular farming of life saving therapeutics in plants
|SHAYAN SARKAR, PhD (2012- 2018) PhD awarded in 2018|
|ABHIMANYU DAS, Laboratory Technician (2010 – Present)|
|DEBASISH DEB, Senior Research Fellow (2014- Present)|
|ANKITA SHRESTHA, Senior Research Fellow (2014- Present)|
|AHAMED KHAN, Senior Research Fellow (2014 – Present)|
JEKY CHANWALA, Senior Research fellow-UGC (2017- Present)
Pearl millet genomics and gene editing
|LINI SETHI, Junior Research fellow (2017- Present)|
|BADRINATH KHADANGA, Project JRF (2018- Present)|
|NOOHI NASIM, Research Associate (2019- Present)|
DR. I. SRIRAM SANDEEP, Research Associate (2019 – Present)
|Deepak Jha, JRF (2019-Present)|
“Studies on interactions of plant transcription factors in promoters from plant infecting pararetroviruses”
Funding Agency: DBT, Govt. of India
Duration: 3 years (2011-14).
Grant Size: 46.87 Lakhs
ndey [at] ils [dot] res [dot] in, nrisinghad [at] yahoo [dot] com
Nalco Square, Bhubaneswar-751023, India
0091 674 2300728
0091 674 2300137/ 2301476 ext. 2010091 674 2300728