Clinical Proteomics & Proteoinformatics

Academic Qualification

1. Ph.D. (Applied Biology): National Institute for Research in Reproductive Health (Indian Council of Medical Research), Mumbai (MS) University of Mumbai.
2. M.V.Sc. (Genetics and Animal Breeding): Bombay Veterinary College, Mumbai Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli (MS).
3. B.V.Sc & A.H (Veterinary Science): Bombay Veterinary College, Mumbai Konkan Krishi Vidyapeeth, Dapoli (MS).
4. PGDIT (Post Graduate Diploma in Information Technology): Symbiosis Centre for Distance Learning (SCDL), Pune (MS)

Academic Positions

1. Scientist: Institute of Life Sciences, Bhubaneswar-751023, India
2. Ph.D. Research Scholar: National Institute for Research in Reproductive Health (Indian Council of Medical Research), Mumbai (MS)
3. Scientist: Molecular Diagnostics and Research Centre, Mumbai (MS)

Membership of Societies

1. Life Member and Executive Council Member (Since Jan 2013), Proteomics Society of India (PSI)
2. Member, Human Proteome Organization (HUPO)
3. Life Member, Laboratory Animals Scientist Association, India (LASA)
4. Life Member, Society of Biological Chemists, India (SBC)
5. Life Member, Indian Society for Mass Spectrometry (ISMAS)
6. Life Member, Indian Virological Society (IVS)

Clinical Proteomics & Proteoinformatics

Broad research activities

Understanding of cancer and viral diseases through clinical proteomics

Our aim is to understand changes in cellular proteome due to various biological/disease conditions using advanced quantitative and targeted proteomics approaches.

At present, our lab is mainly focused on cancers (such as Nasopharyngeal Carcinoma, cervical/ovarian cancer) and various viral diseases (such as Chikungunya, Dengue,  Rabies, COVID-19). We are interested to add many more diseases which are more relevant in this geographical region.

Research focus:

• Disease Proteome Mapping
• Bio-marker discovery in various clinical diseases
• To decipher the role of important protein/s in disease pathogenesis
• Identification and characterization of post-transnational modifications (PTMs) with respect to their role in disease pathogenesis

Our group is working on identifications and Characterization of Bio-markers in various Diseases, identification of PTMs and their role in disease pathogenesis using high throughput mass spectrometry-based proteomics approaches. We used various advanced proteomics approaches such as gel-based, non-gel-based, labeled, labeled free proteomics approaches in our experiments.

Clinical Proteomics & Proteoinformatics

Selected Publications

1. Behera S., Pharande R. R., Reddy R. R., Majee S. B., Mukherjee S., and Suryawanshi A. R.*. (2020). Quantitative proteomics leads to identify dog brain proteins involved in rabies virus infection: Implication in understanding viral pathophysiology.  Journal of proteins and proteomics 11, 241–257. https://doi.org/10.1007/s42485-020-00051-w.
2. Suresh V, Mohanty V, Avula K, Ghosh A, Singh B, Reddy RK, Parida D, Suryawanshi AR, Raghav SK, Chattopadhyay S, Prasad P, Swain RK, Dash R, Parida A, Syed GH, Senapati S. (2021). Quantitative proteomics of hamster lung tissues infected with SARS-CoV-2 reveal host factors having implication in the disease pathogenesis and severity. FASEB J. Jul;35(7):e21713. doi: 10.1096/fj.202100431R. PMID: 34105201.
3. Das P, Badhe MR, Sahoo PK, Reddy RRK, Suryawanshi AR, Mohanty J. Immunoproteomic analysis of fish ectoparasite, Argulus siamensis antigens. Parasite Immunol. (2021) Apr 2:e12837. doi: 10.1111/pim.12837. Epub ahead of print. PMID: 33811350. (Impact factor: 2.05)
4. Chatterjee K, De S, Deb Roy S, Sahu SK, Chakraborty A, Ghatak S, Das N, Mal S, Roy Chattopadhyay N, Das P, Reddy RR, Mukherjee S, Das AK, Puii Z, Zomawia E, Singh YI, Tsering S, Riba K, Rajasubramaniam S, Suryawanshi AR, Choudhuri T. (2021) BAX -248 G>A and BCL2 -938 C>A Variant Lowers the Survival in Patients with Nasopharyngeal Carcinoma and Could be Associated with Tissue-Specific Malignancies: A Multi-Method Approach. Asian Pac J Cancer Prev. Apr 1;22(4):1171-1181. doi: 10.31557/APJCP.2021.22.4.1171. PMID: 33906310. (Impact factor: 1.58).
5. Raghav S*, Ghosh A, Turuk J, Kumar S, Jha A, Madhulika S, Priyadarshini M, Biswas VK, Shyamli PS, Singh B, Singh N, Singh D, Datey A, Kiran A, Smita S, Sabat J, Bhattacharya D, Kshatri JS, Vasudevan D, Suryawanshi AR, Dash R, Senapati S, Beuria TK, Swain R, Chattopadhyay S, Syed GH, Dixit A, Prasad P, Pati S, Parida A*. (2020). Analysis of Indian SARS-CoV-2 genomes reveal prevalence of D614G mutation in Spike protein eliciting increase in interaction with TMPRSS2 and virus infectivity. Frontiers in Microbiology (accepted on 21st October 2020, In press) (Impact factor: 4.23)
6. Roy Chattopadhyay N, Chatterjee K, Tiwari N, Chakrabarti S, Sahu SK, Deb Roy S, Ghosh A, Reddy RR, Das P, Mal S, Karnar BB, Das AK, Tsering S, Riba K, Puii Z, Zomawia E, Singh YI, Suryawanshi AR, Kumar A, Ganguly D, Goswami C, Choudhuri T. TLR9 Polymorphisms Might Contribute to the Ethnicity Bias for EBV-Infected Nasopharyngeal Carcinoma. iScience. 2020 Mar 27;23(3):100937. doi: 10.1016/j.isci.2020.100937. PMID: 32179470 (Impact factor: 4.4)
7. Mohanty J, Sahoo S, Badhe MR, Pillai BR, Sahoo PK, Suryawanshi AR, Patnaik BB. Lectin-Like Activity of Hemocyanin in Freshwater Prawn, Macrobrachium rosenbergii. Protein J. 2020 Aug;39(4):358-365. doi: 10.1007/s10930-020-09912-1. PMID: 32661731(Impact factor: 1.3)
8. Kakar-Bhanot R, Brahmbhatt K, Kumar V, Suryawanshi AR, Srivastava S, Chaudhari U, Sachdeva G. Plasma membrane proteome of adhesion-competent endometrial epithelial cells and its modulation by Rab11a. Mol Reprod Dev. 2019 Nov 18. doi: 10.1002/mrd.23292. [Epub ahead of print] PubMed PMID: 31737970 (Impact factor: 3.113)
9. Roy Chattopadhyay N, Chakrabarti S, Chatterjee K, Deb Roy S, Kumar Sahu S, Reddy RR, Das P, Bijay Kanrar B, Kumar Das A, Tsering S, Puii Z, Zomawia E, Singh YI, Suryawanshi A, Choudhuri T. Histocompatibility locus antigens regions contribute to the ethnicity bias of Epstein-Barr virus-associated nasopharyngeal carcinoma in higher-incidence populations. Scand J Immunol. 2019 Oct;90(4):e12796. doi: 10.1111/sji.12796. PubMed PMID: 31145476 (Impact factor: 2.563).
10. Mukherjee S, Sengupta N, Chaudhuri A, Akbar I, Singh N, Chakraborty S, Suryawanshi AR, Bhattacharyya A, Basu A. PLVAP and GKN3 Are Two Critical Host Cell Receptors Which Facilitate Japanese Encephalitis Virus Entry Into Neurons. Sci Rep. 2018 Aug 6;8(1):11784. doi: 10.1038/s41598-018-30054-z.
11. Sahu SK, Chakrabarti S, Roy SD, Baishya N, Reddy RR, Suklabaidya S, Kumar A, Mohanty S, Maji S, Suryanwanshi A, Rajasubramaniam S, Asthana M, Panda AK, Singh SP, Ganguly S, Shaw OP, Bichhwalia AK, Sahoo PK, Chattopadhyay NR, Chatterjee K, Kundu CN, Das AK, Kannan R, Zorenpuii, Zomawia E, Sema SA, Singh YI, Ghosh SK, Sharma K, Das BS, Choudhuri T. Association of p53 codon72 Arg>Pro polymorphism with susceptibility to nasopharyngeal carcinoma: evidence from a case-control study and meta-analysis. Oncogenesis. 2016 May 9;5:e225. doi: 10.1038/oncsis.2016.31. PubMed PMID: 27159678 (Impact factor: 3.952).
12. Sengupta N, Mukherjee S, Tripathi P, Kumar R, Suryawanshi AR, Basu A. Cerebrospinal Fluid Biomarkers of Japanese Encephalitis [version 2; referees: 3 approved]. F1000Research 2015, 4:334 (doi: 10.12688/f1000research.6801.2).
13. Swaroop S, Sengupta N, Suryawanshi AR, Adlakha YK, Basu A. HSP60 plays a regulatory role in IL-1β-induced microglial inflammation via TLR4-p38 MAPK axis. J Neuroinflammation. 2016 Feb 2;13(1):27. doi: 10.1186/s12974-016-0486-x. (Impact factor: 5.408)
14. Kumar S, Mamidi P, Kumar A, Basantray I, Bramha U, Dixit A, Maiti PK, Singh S, Suryawanshi AR, Chattopadhyay S, Chattopadhyay S. Development of novel antibodies against non-structural proteins nsP1, nsP3 and nsP4 of chikungunya virus: potential use in basic research. Arch Virol. 2015 Nov;160(11):2749-61. doi: 10.1007/s00705-015-2564-2. (Impact factor: 2.390)
15. Pratheek BM, Suryawanshi AR, Chattopadhyay S, Chattopadhyay S. In silico analysis of MHC-I restricted epitopes of Chikungunya virus proteins: Implication in understanding anti-CHIKV CD8(+) T cell response and advancement of epitope based immunotherapy for CHIKV infection. Infect Genet Evol. 2015 Apr;31:118-26. doi: 10.1016/j.meegid.2015.01.017.
16. Kumar A, Mamidi P, Das I, Nayak TK, Kumar S, Chhatai J, Chattopadhyay S, Suryawanshi AR, Chattopadhyay S. A novel 2006 Indian outbreak strain of chikungunya virus exhibits different pattern of infection as compared to prototype strain. PLoS One. 2014 Jan 20;9(1):e85714. doi: 10.1371/journal.pone.0085714. eCollection 2014 Jan 20.
17. Chattopadhyay S, Kumar A, Mamidi P, Nayak TK, Das I, Chhatai J, Basantray I, Bramha U, Maiti PK, Singh S, Suryawanshi AR, Chattopadhyay S. Development and characterization of monoclonal antibody against non-structural protein-2 of Chikungunya virus and its application. J Virol Methods. 2014 Jan 21;199C: 86-94. doi: 10.1016/j.jviromet.2014.01.008.
18. Joshi CS, Suryawanshi AR, Khan SA, Balasinor NH, Khole VV. Liprin α3: a putative estrogen regulated acrosomal protein. Histochem Cell Biol. 2013 Apr;139 (4):535-48.
19. Suryawanshi AR, Khan SA, Joshi CS, Khole VV. Epididymosome Mediated Acquisition of MMSDH an Androgen Dependent and Developmentally Regulated Epididymal Sperm Protein. J Androl. 2012 Sep-Oct;33(5):963-74.
20. Gajbhiye RK, Sonawani A, Khan SA, Suryawanshi AR, Kadam S, Warty N, Raut V, Khole VV. Identification and validation of novel serum markers for early diagnosis of endometriosis. Hum Reprod. 2012 Feb;27(2):408-17.
21. Khan SA, Jadhav SV, Suryawanshi AR, Bhonde GS, Gajbhiye RK, Khole VV (2011). Evaluation of Contraceptive Potential of a Novel Epididymal Sperm Protein SFP2 in a Mouse Model. Am J Reprod Immunol. 2011 Sep;66(3):185-98.
22. Suryawanshi AR, Khan SA, Gajbhiye RK, Gurav MY, Khole VV (2010). Differential Proteomics Leads to Identification of Domain Specific Epididymal Sperm Proteins. J Androl. 2011; 32(3):240-59.
23. Khan S A*, Suryawanshi A R*, Ranpura S, Jadhav S, Khole V V. (2009). Identification of Novel Immunodominant Epididymal Sperm Proteins using Combinatorial Approach. Reproduction, 2009; 138: 81-93 (*Authors have equally contributed)
24. Gajbhiye R K, Suryawanshi A R, Khan S A, Meherji P K, Warty N R, Raut V S, Chehna N A, Khole V V. (2008). Multiple endometrial antigens are targeted in autoimmune endometriosis. Reprod Biomed Online.; 16 (6):817-24.
25. Suryawanshi A R., Kanadkhedkar H L., and Umrikar U D. (2004). Chromosome Analysis of Domestic Dogs. Indian Vet J.; 81:1071-1073.

Access full publication list: ‪Dr. Amol R Suryawanshi‬ – ‪Google Scholar‬

Clinical Proteomics & Proteoinformatics

Dr. Amol Ratnakar Suryawanshi (PI)

My lab Members:  Group photo-2019

Present Lab Members:

• Ms. Suchismita Behera
• Ms. Rohila Jha
• Mr. Narottam Manna
• Mr. R. Rajendra Kumar Reddy

Ms. Suchismita Behera, SRF

Ms. Rohila Jha, JRF

Mr. Narottam Manna, JRF

Mr. R. Rajendra Kumar Reddy, Lab Technician (Proteomics Facility Manager)

Clinical Proteomics & Proteoinformatics

Current /Past Projects

Post Doctoral fellows/Research Associate/N-PDF:

My laboratory is looking for prospective enthusiastic Post Doctoral fellows/Research associates, who are interested in the field of clinical proteomics, metabolomics and related areas. The candidates who have finished/finishing their Ph.D. with experience in proteomics, molecular/cell biology and handling omics data will be prefered. The candidates should have a valid postdoctoral fellowship from any funding agency (DBT/CSIR/ICMR/SERB etc.) or interested to apply for the fellowship in the in above mentioned research areas are encouraged to apply with their detailed CV, one page write-up describing their research interests and One/two page brief concept note of proposed research work to amol@ils.res.in or amolsurya@gmail.com .

I will be happy to guide prospective candidates through the research proposal and application process for obtaining Postdoc fellowships.

Ph.D. students:

Prospective Ph.D. students please refer to the Ph.D. Programme advertisement on ILS website for details.

We are looking for dedicated, meticulous, hard-working and calm student who is willing to accept the challenges to persue the research dream.

The candidates completed/completing their masters/post graduate degree and wish to pursue PhD in the field of clinical proteomics for understanding cancer and viral diseases may write to amol@ils.res.in or amolsurya@gmail.com along with your CV and research interest. Preference will be given to the student having valid CSIR/UGC/DBT/ICMR JRF or any other national fellowship.

Summer dissertation:

Students interested for summer dissertation, please refer to https://www.ils.res.in/ils-training-programme/ for details.

Students who wish to pursue summer trainees (minimum 2 months) and Master’s project (minimum 6 months) may write to amol@ils.res.in or amolsurya@gmail.com along with your CV and research interest.