Vibrio cholerae, Staphylococcus aureus and bacterial pathogenesis

Education:  

Ph.D. Thesis: Studies on certain virulence properties of Aeromonas hydrophila.

Academic positions: 

Scientist E, (June 2, 2007- contd.); Scientist D/Reader, (June 2, 2003-June 1, 2007.), Institute of Life Sciences, Bhubaneswar-751023, India;

Scientist C, (November 4, 1997-May 31, 2003); Scientist E-I, (August 28, 2000-November 30, 2002, on deputation); Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram–695014, India;

Assistant Professor, (February 17, 1997–October 31, 1997), SBSPG Institute of Biomedical Sciences and Research, Dehradun–248161, India;

Senior Research Associate, (August 1, 1996–February 16, 1997); Research Associate, (April 4, 1991–May 31, 1996); Senior Research Fellow and Junior Research Fellow, (March 1, 1986–February 28, 1991); Research Fellow, (September 17, 1985–February 28, 1986), Department of Microbiology, Institute of Medical Sciences. Banaras Hindu University, Varanasi – 221 005, India 

 Training: 

1.     September 29, 2008- November 21, 2008, Microarray based analysis of Vibrio cholerae (Supervisor: Prof. Rita R. Colwell), Maryland Pathology Research Institute, University of Maryland, College Park, MD

2.     August 5, 2002-September 13, 2002: PCR, gene cloning, expression, purification of protein, fermentation, mice immunization and subsequent challenge and survival study (Supervisor: Prof. Dr. G.S. Chhatwal, Department of Microbial Pathogenesis and Vaccine Research, GBF, Mascheroder Weg 1, 38124 Braunschweig, Germany).

3.     December 12, 2000-February 6, 2001: Monoclonal antibody production using Hybridoma technique and tissue culture assay (Supervisor: Dr. T. Ramamurthy, National Institute of Cholera & Enteric Diseases, Calcutta).

4.     March 22, 1998-July 12, 1998: Isolation and purification of plasmid DNA, homologous recombination, Colony and Western Hybridization (Supervisor: Prof. James B. Kaper, Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland).

5.     February 3, 1998-March 21, 1998: Molecular Biology including isolation and purification of chromosomal DNA, PCR, AFLP, RFLP, PFGE, Ribotyping, Dot-Slot, and Southern Hybridization (Supervisor: Prof. Rita R. Colwell, Center of Marine Biotechnology, UMBI, Baltimore, Maryland).  

Honors and awards:  

1. ASM Indo-US Visiting Research Professorship to visit Maryland Pathology Research Institute, University of Maryland, College Park, MD
2. GBF International Training Program in Infection Biology on Gene to Vaccine to visit German Research Centre for Biotechnology, Braunschweig, Germany.
3. Cold Spring Harbor Laboratory Course on Advanced Bacterial Genetics at Delbruck Laboratory, CSHL, NY, USA from June 7-27, 2000. (Not availed).
4. Visiting Scientistshi of Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, MD, USA.
5. UNESCO Short – Term Training Fellowship in Biotechnology, Paris, France.
6. Senior Research Associateship (Pool Officership) of Council of Scientific and Industrial Research, New Delhi, India
7. Research Associateship of Council of Scientific and Industrial Research, University Grants Commission, Indian Council of Medical Research, New Delhi, India
8. Junior and Senior Research Fellowships of Council of Scientific and Industrial Research, New Delhi, India
9. AMI Best poster award in Medical and Veterinary Microbiology session on paper entitled "Molecular characterization of clinical and environmental isolates of Vibrio cholerae O1 and O139" by DV Singh, G Matte, M Matte, BN Shukla, SC Sanyal, A Huq and RR Colwell presented at 39th Annual Conference of AMI at Mangalore from Dec 5-6, 1998.

Fellowships:

1. Member, New York Academy of Sciences, New York (Until 2003)
2. Fellow Biotech Research Society of India

Membership of Professional Associations / Societies: 

1. Life Member:          Association of Microbiologist of India

2. Life Member:          Indian Immunology Society

3. Life Member:          Indian Association of Medical Microbiologists

4. Founder Member :  Biotech Research Society of India

5. Full Member:          American Society for Microbiology 

Reviewer of Scientific Journals: 

1. Current Science published by Current Science Association, India
2. Environmental Research, FEMS Microbiology Letters and FEMS Microbiology Ecology published by Elsevier Science B.V.
3. Journal of Health Population and Nutrition, ICDDR-B, Dhaka
4. Clinical Chemistry published by American Association for Clinical Chemistry
5. Applied Biochemistry and Applied Biotechnology
6. Indian Journal of Medical Microbiology published by IAMM, India
7. Journal of Medical Microbiology published by SGM, UK
8. Journal of Microbiology, published by Korean Society of Microbiology, Korea

Reviewer of Research Projects: 

Department of Science and Technology, Health Science Division, New Delhi

Extramural Research Projects:

1. Ocular infections caused by Staphylococcus species: Study of clinico-microbiologic features, virulence markers and genome profile, DST, New Delhi (2009-2012). PI: Dr. DV Singh, ILS, Bhuabneswar; PI: Dr. Savitri Sharma, LVEI, Bhubaneswar
2. Molecular analysis of environmental Vibrio cholerae strains: relationships with clinical isolates, DST, New Delhi (2007-2010).
3. Purification and characterization of a new toxin produced by candidate cholera vaccine strains, ICMR, New Delhi (2005-2008).
4. Characterization of the new secretogenic toxin produced by Vibrio cholerae non-O1, non-O139 using panel of monoclonal antibodies. PI: Dr. DV Singh; PCo-I: Dr. GB Nair, DST, Government of India, New Delhi (2000-2003).
5. DST Young Scientist project: One (Not availed).